Scientific Program

Day 1
Day 2

Keynote Session:

10:30-11:10

Nick Kostovic

President Kostovic Acupuncture by bio Electrons Laser, Corp

Title: Bio Electron's Laser Acupuncture by KBTE Medical laser Device

Biography:

Nick Kostovic was born on December 6, 1950 in city Split, region Dalmatia, currently Croatia. I graduated from Split Gymnasium in 1969 with an Associate of Arts Degree in Humanities and Science. I am Italian and US citizen.

Abstract:

The K-BTE device releases electrons, enriched with natural acids, and is capable of “melting by burning off,” number of sick cells with no harm to the healthy cells.then dispersing, any number of benign or malignant tumors with metastasis in the brain or any other physical organ – with no harm to healthy cells.It “Melts by burning off” then disperses dead cells from the fiber of atrophied muscle, bone and cartilage tissue – as well as plaque from the vascular system – with no harm to healthy cells.The K-BTE device attracts and transfers light – which consists of elemental bio electrons’ photons and electron neutrinos – into the brain. This fosters the

regeneration, recovery and re-growth of neurons in the brain, thereby regaining

its ability to send impulses throughout the body while improving mobility.

Melting and destroying malignant cancer cells in the brain – or any other physical organ – accomplished with no side effects and no harm to healthy cells.

Melting cleanses internal plaque from the vascular system in the brain or any other physical organ. This will prevent strokes, heart attacks, diabetes and many different neurological disorders.

Melting cleanses the fiber tissue of dead cells – dispersing them and making space for the formation of new and healthy cells. This is the most important step for a vital, longer lasting life.The K-BTE device has the capability to cleanse the body of radioactive radiation particles as well as different types of toxic biochemical nerve gas. This enriched-electrons-radiation-emitting-process is absolutely non-radioactive, non-toxic and is, in no way, “shock therapy.”

11:30-12:10

Ari Y. Baratz

MD, FRCSC (REI) In CReATe Fertility centre, Canada

Title: Naturopathic Medicine in Oncology

Biography:

Ari Y. Baratz is an active member of the College of Physicians & Surgeons of Ontario, the Royal College of Surgeons of Canada, the Society of Obstetricians & Gynecologists of Canada, the American College of Obstetricians & Gynecologists, the Canadian Fertility & Andrology Society, and the American Society of Reproductive Medicine. Dr. Baratz is an Executive Board member for Women’s College Hospital, Medical Staff Association, the Women’s College Hospital Alternate Funding Plan, and is Chair for the Ontario Medical Association Reproductive Biology Section.

Abstract:

Last years have witnessed major advancement in the field of ART and improvement in its outcome. Some modalities were proven to be effective and others were not. The goal of the current presentation is to to assess and present the evidence for both established and emerging approaches for optimization of ART outcome. Pre-cycle evaluation and possible procedures suggested for improving the outcome will be discussed. Dealing with hydrosalpinx and endometrioma before ART will be highlighted in accordance to latest evidence. Individualized ovarian stimulation and decision making during different times of the stimulation cycle, whether on the day of oocyte triggering or the day of embryo transfer will be addressed. Additionally, the value of new modalities suggested for improving the ART outcome will be highlighted in according to the latest evidence and committee opinions.

12:10-12:50

Bruce D. Murphy

Faculty of veterinary medicine, University of Montreal, Canada

Title: Treatment with metformin and combination of metformin plus pioglitazone on serum levels of IL-6 and IL-8 in polycystic ovary syndrome: A randomized, clinical trial.

Biography:

Bruce D.Murphy's laboratory has focused on the role of orphan nuclear receptors of the NR5A family in regulation of reproductive events. We have shown that NR5A2, aka liver receptor homolog-1, is essential for the processes of ovulation and luteal function. Its expression in the uterus is likewise necessary for establishment of gestation. NR5A1, aka steroidogenic factor-1, is necessary for maturation of ovarian follicles. Our current studies are aimed at exploring the multiple mechanisms by which NR5A1 and NR5A2 regulate ovarian events, including proliferation, differentiation and cytoskeletal remodeling. Our investigations are characterized by phenotypic analysis of targeted mutations in mice combined with global approaches to determining the widespread molecular changes that occur associated with depletion of the NR5A genes.

Abstract:

Statement of the problem: Pancreatic cancer remains one of the deadliest cancers despite much progress in the science of tumour molecular characterisation and treatment strategies. The introduction of targeted treatments and immunotherapies has yet to provide desirable effect comparable with other cancers such as breast cancer and melanoma. This can be attributed to a pancreatic cancer lower immune-sensitivity and the ability to successfully evade the surveillance of immune system, particularly having resistance against lethal effects of cytotoxic T lymphocytes (CTLs). Tumour-immune resistance is mediated by tumour immune-modulators (TIM) that engage immune-inhibitory receptors on CTLs. Although past research has identified several interaction mechanisms, there is no single study that clearly delineates the molecular dynamics taking place between pancreatic tumour cells and CTLs. Elucidating pancreatic cancer molecular mechanism of immune-evasion and immune-resistance has the potential of identifying downstream onco-targets regulated by TIM and the possibility of developing more effective therapeutic agents.

Methodology & Theoretical Orientation: Exome and transcriptome sequencing of pancreatic tumour cells (from patient samples) and 6 pancreatic cell lines will be performed before and after exposure to chemo- and immune-therapeutics to identify genomic and expression patterns of sensitive and resistant tumour cells. Downstream targets regulated by TIM will be characterised by MudPIT (Multidimensional Protein Identification Technology). Results validation will be obtained using gene silencing shRNA assays for selected oncotargets, followed by exposure to chemo- and immune-therapeutics. Various bioinformatics tools and databases will be used to analyse exome and transcriptome sequence data. Additionally, evaluation of in-silico bioinformatics tools for functional variomics and network perturbation will be performed with the aim to identify best genotype-phenotype connection/prediction algorithms.

Expected Outcomes: this study is expected to provide insight to the role of pancreatic tumour immune mediators (TIM) in driving immune inhibitory effects of cytotoxic T-lymphocytes (CTL). The findings will help in understanding how pancreatic cancer drives immune evasion and in identification of onco-targets for drug development. Conclusion and Significance: The outcomes of the study have the potential of Improving prognosis and clinical outcomes in patients with pancreatic cancer through the development of new therapeutic approaches and strategies.

Oral Session 1:

Rreproductive| (PCOS) | Medicine | Toxicology | Endocrinology

Chair

Ben Fisher

Director, Division of Reproductive, Gastro-Renal, and Urological Devices, ODE, CDRH

Co-Chair

Nick Kostovic

President Kostovic Acupuncture by bio Electrons Laser

14:00-14:25

Gregory Lee

University of British Columbia, Canada

Title: Cancerous immunoglobulins and potential clinical applications

Biography:

Gregory Lee completion of his PhD degree in Biophysical Chemistry at California Institute of Technology in 1972, he joined UCSD as a NIH Postdoctoral Fellow to carry out pioneer work on general ligand affinity chromatography. In 1976, he studied enzymology and biochemical genetics at the National Institutes of Health and in 1981, he was appointed as a faculty member and director of the Andrology Laboratory at the University of British Columbia. During the last two decades, he has created numerous monoclonal antibodies for diagnostic and therapeutic applications in human health care, including RP215 and GHR106 for potential use as anti-cancer drugs.

Abstract:

During the last decade, substantial research has been performed to explore the expression and role of cancerous antigen receptors, such as immunoglobulins and T cell receptors, compared with those of normal immune cells. These cancerous immunoglobulins differ from immune cell-derived immunoglobulins by the presence of a unique carbohydrate-associated epitope recognized by RP215 monoclonal antibody. CA215 is a group of glycoproteins, with this RP215-specific epitope, and consists of antigen receptors, and other members of the immunoglobulin superfamily. Through numerous biological and immunological studies, it was generally concluded that the expression of cancerous immunoglobulins is essential for the growth and proliferation of cancer cells in vitro and in vivo. Furthermore, through gene regulation studies, the respective effects of RP215 monoclonal antibody against CA215, and anti-antigen receptor antibodies on a dozen of selected genes which are expressed in cancer cells, such as NFκB-1, IgG, P21, cyclin D1, ribosomal P1, and c-fos, were found to be highly correlated. In particular, the expressions of toll-like receptors (TLR-2, -3, -4, -6, -7 and -9), which are involved in the innate immunity of cancer cells, were similarly altered in treatments with RP215 and with anti-antigen receptor antibodies. For example, RP215 and anti-antigen receptor antibodies were found to both up-regulate TLR-2 and/or TLR-3 and down-regulate TLR-4 and TLR-9 in cancer cells. Based on these studies, it is reasonable to postulate that cancerous immunoglobulins are highly involved in or modulate the innate immune system to allow the growth and survival of cancer cells within the human body.

14:25-14:50

Evert Ketting

Faculty Guttmacher Institute

Title: Current status of sexuality education in Europe

Biography:

Evert Ketting is an international specialist in sexual and reproductive health and rights. Since 1989, he has worked as an international expert for various organizations and governmental agencies—including the International Planned Parenthood Federation, European Union, World Health Organization (WHO), United Nations Population Fund, United Nations Educational, Scientific and Cultural Organization (UNESCO), United Nations Children’s Fund (UNICEF) and the World Bank—in more than 25 countries in Eastern Europe, Central Asia and Africa. Currently, he is also a senior researcher at Radboud University Nijmegen Medical Centre, where he has worked on a UNESCO project on the cost and cost-effectiveness of sexuality education in six countries in Asia, Africa and Europe, among others. In the past few years, he played a leading role in the WHO Regional Office for Europe and the Federal Centre for Health Education’s initiative to develop “Standards for Sexuality Education in Europe” (2010) and the related “Guidance for Implementation” (2013). Dr. Ketting obtained his PhD in Social Sciences from Utrecht University.

Abstract:

School sexuality education has a long history in Europe, starting in Sweden, where it became a mandatory teaching subject in 1954. Since then it was also adopted in most other European countries; first in Western and Northern Europe and later on also in several Southern and Eastern European countries. In 2008, the German Federal Health Education Centre (BZgA, a WHO collaborating centre), initiated closer international collaboration in the field of sexuality education at the European level. To this end, it organised a “European Expert Group on Sexuality Education”, which is still active in improving, promoting, and in exchanging information and experience in this field. Since 2010, this expert group has released various documents on the subject, the influential “Standards for Sexuality Education in Europe (2010)”, that has been used in many European countries for the development of sexuality education curricula and for integrating the subject in school educational programmes. In 2016, in collaboration with the European Network of IPPF, BZgA initiated a study on the current status of sexuality education in Europe (including Central Asia), in which a representative selection of 24 European (and Central Asian) countries participated. The initial results of this unique study will be presented at an international conference on sexuality education in Europe, in Berlin in May 2017, and the full results are scheduled for publication a few months later. The study provides a detailed assessment of the current status of sexuality education in Europe. It is now a mandatory teaching subject in schools in the vast majority of European countries. Unlike in other world regions, the teaching in about half of the European countries has a comprehensive (or holistic) character, is fully integrated and spread out throughout school curricula, starts at young ages and lasts for several years, is based on human rights, and starts from a positive approach to sexuality.

14:50-15:15

Jean-Marie Poffe

Pharmacien specialiste, en micro-nutrition chez consultation de Auicronutrition , Belgium

Title: G.I.T IMMUNOLOGY

Biography:

Jean Marie Poffe is a pharmacist since 20 years and completed his pharmacist diploma in 1976. He received a certificate of pharmacist specialization in Clinical Bacteriology. He has also done specialization in nutrition and micro nutrition and works as a nutrition consultant at Nutrition Cellulaire, Belgium.

Abstract:

We will First of all start with a historical reminder of the membrane of the G.I.T and its constituents Then we will discuss the G.I.T an immunological barrier = the G.A.L.T

We will pass on the consequences of civilization diseases. (intolerance, allergies, M.I.C...) Next, we will talk about the immune responses the differentiation of lymphocyte and secretion of IgA, autoimmune diseases, the interest of prebiotics and probiotics and the immunity.

And for the conclusion: << Captial importance of the proper functioning of the G.I.T for the well being of our patients.

15:15-15:40

Dalida Omar Badla

Damascus University, Faculty of Medicine, Syria

Title: Women Cancer Risk and Polycystic Ovary Syndrome (PCOS)

Biography:

Dalida Omar Badla has completed MBBS from faculty of medicine of Damascus University-Syria in 1990 and had certificate of specialist registration in ob&gyn from Ministry of Health OF Syria in 1994 then Master degree (MD)from University Hospital of Aleppo-Syria in 1995 .Syrian Board in 2015 .Diploma in Minimal Accss Surgery D.MAS from World Association of Laprascopic Surgeons in 2017.Fellowship in Assisted Reproductive Technology from International Association of Assisted Reproductive Technology at WLH,NCR DELHI in 2017 . She is specialist of Obstetric and Gynaecology at Danat Al Emarat hospital for Women and Child Health in Abu Dhabi, UAE

Abstract:

Polycystic ovary syndrome (PCOS) is a complex endocrine disorder characterized by oligomenorrhea, hyperandrogenism, and polycystic ovariesshape by ultrasound.

It has an estimated prevalence of 4–21% in reproductive aged women depending on the diagnostic criteria and population examine. PCOS is associated with metabolic abnormalities including dyslipidemia, insulin resistance, and type II diabetes and is also one of the most common causes of reduced fertility . The altered metabolic and hormonal environment among women with PCOS may increase their risk of some types of cancer.like: Endometrial cancer which explained by the absence of ovulation and producing estrogen, but not progesterone, which causes the endometrium (the lining of the uterus) to grow too much and undergo atypical cell changes. This is a pre-cancerous condition called endometrial hyperplasia. If the thickened endometrium is not treated, over a long period of time it can develop into endometrial cancer, and Ovarian cancer: asPCOS increased androgen exposure presence of androgen receptors on normal ovarian cells as well as benign and borderline tumors, and a doubling of androgen levels during pregnancy is associated with a 40–50% increased risk of borderline serous and invasive. Breast cancer:Characteristics and consequences of PCOS have been previously associated with both increased and decreased risk of breast cancer. increased levels of insulin in the blood can increase the risk of formation of breast cancer cells.and Other kinds of cancers might linked to Also PCOS medications that may influence cancer risk such as : Oral contraceptives, Metformin, Ovulation-induction therapies.

15:40-16:05

Eman A Elgindy

Professor of Obstetrics and Gynecology, Zagazig University School of Medicine, Egypt

Title: Optimization of ART Outcome: Updated Evidence and Best Practice

Biography:

Eman Elgindy is a consultant in reproductive medicine, professor Obstetrics and Gynecology, Zagazig University and Clinical Director of Rahem fertility center, Zagazig, Egypt. She obtained her MD in 2001 from Zagazig University and PhD in reproductive Medicine in 2013 from Maastricht University. Dr Elgindy authored or co-authored many research studies published in both national and international journals (Highest ranked journals like, Green Journal, Human Reproduction journal, Fertility and Sterility journal and Reproductive Biomedicine Online journal). Her research interests include ovarian reserve, optimizing controlled ovarian stimulation protocols and fertility preservation. She has many presentations in national and international conferences. Dr Elgindy is a member of MEFS board from 2013 until 2017 and the editor of MEFS news from 2014 till now. In 2008 she received the Middle East Fertility Society Research Award for the best research in the field of human reproduction. The University of Zagazig offered Dr Elgindy the Certificate of Distinction in the field of medical sciences in 2012.

Abstract:

16:25-16:50

Kerri King-Hurley

Shore Institute for Reproductive Medicinel

Title: HIV infected persons in serodiscordant relationships: a preliminary study from a Jamaican cohort

Biography:

Shore Institute for Reproductive Medicinel, USA

Abstract:

Oocyte, embryo and ovarian tissue cryopreservation are being increasingly proposed for fertility preservation among cancer patients undergoing therapy to enable them to have babies after the cancer is cured. Embryo cryopreservation is not appropriate for single girls without any spermpartner. It is impossible in cases requiring immediate cancer cure because oocyte retrieval is an extended procedure. Thus ovarian tissue cryopreservation has been suggested for fertility preservation especially in cancer patients. The main goal of ovarian cryopreservation is re-implanting the tissue into the body to restore fertility and the hormonal cycle. Different cryopreservation protocols have been examined and established for vitrification of biological samples. We have used Cryopin to plunge ovarian tissue into the liquid nitrogen and promising results have been observed. The possibility of recurrence of malignancy in the reimplanted tissue could be a problem. Xenografting-implantation of the preserved tissue in another species-also has its drawbacks such as molecular signaling from the recipient. In vitro follicle culturing is a safer method to obtain mature oocytes for fertilization and the various studies that have been carried out in this area are reviewed in this paper.

16:50-17:1

Lisa Oliver

University of Nantes, France

Title: Current status of sexuality education in Europe

Biography:

Lisa Oliver has completed her PhD at University of Paris 7. She is currently working in the group of Dr. François Vallette at the CRCINA, INSERM-University of Nantes. She has published more than 40 papers in reputed journals.

Abstract:

Glioblastoma multiforme is the most common brain cancer in adults. Radiotherapy is the most effective post-operative treatment for the patients even though gliomas are considered one of the most radio-resistant tumors. As a consequence there is rapid recurrence of the tumor probably due to the presence of cancer stem cells (CSC), which are radio-resistant. Indeed, the dying irradiated tumor bulk would activate caspase-dependent pathways causing the release of growth-promoting factors that would mobilize and recruit CSC. One of these pathways is the Ca2+-dependent phospholipase A2, the activation of which increases the synthesis and release of arachidonic acid from apoptotic cells and the consequent release of prostaglandin E2 (PGE2). We have evaluated the role of PGE2 in glioma radio-resistance. We used an in-vitro approach using 3D primary cultures derived from representative glioma patients. We show that irradiated glioma cells produced and released PGE2 in important quantities independently of the induction of cell death. We demonstrate that the addition of PGE2 enhances cell survival and proliferation though its ability to trans activate the Epithelial Growth Factor receptor (EGFR) and to activate β-catenin. Indeed, PGE2 can substitute for EGF to promote primary cultures survival and growth in vitro and the effect is likely to occur though the prostaglandin E2 receptor EP2.

Dalida Omar Badla

Damascus University, Faculty of Medicine, Syria

Title: Women Cancer Risk and Polycystic Ovary Syndrome (PCOS)

Biography:

Dr. Dalida Omar Badla has completed MBBS from faculty of medicine of Damascus University-Syria in 1990 and had certificate of specialist registration in ob&gyn from Ministry of Health OF Syria in 1994 then Master degree (MD)from University Hospital of Aleppo-Syria in 1995 .Syrian Board in 2015 .Diploma in Minimal Accss Surgery D.MAS from World Association of Laprascopic Surgeons in 2017.Fellowship in Assisted Reproductive Technology from International Association of Assisted Reproductive Technology at WLH,NCR DELHI in 2017 . She is specialist of Obstetric and Gynaecology at Danat Al Emarat hospital for Women and Child Health in Abu Dhabi, UAE

Abstract:

Polycystic ovary syndrome (PCOS) is a complex endocrine disorder characterized by oligomenorrhea, hyperandrogenism, and polycystic ovariesshape by ultrasound.

Keynote Session:

10:10-10:4

Sergey V. Suchkov,

Director Sechenov University, Russia

Title: Personalized and Precision Medicine as a Unique Healthcare Model through the View of Biodesign and Translational Armamentarium of the Newest Generation.

Biography:

Sergey Suchkov was born in the City of Astrakhan, Russia, in a family of dynasty medical doctors. In 1980, graduated from Astrakhan State Medical University and was awarded with MD. In 1985, maintained his PhD as a PhD student of the I.M. Sechenov Moscow Medical Academy and Institute of Medical Enzymology, USSR Academy of Medical Sciences, Moscow, Russia. In 2001, finished the PostDoc Research Fellowship Program and maintained his Doctor Degree at the National Institute of Immunology, Russia. From 1985 through 1987, worked at Inst of Med Enzymology, USSR Academy of Medical Sciences. From 1987 through 1989, was a senior Researcher, Koltzov Inst of Developmental Biology, USSR Academy of Sciences. From 1989 through 1995, was being a Head of the Lab of Clinical Immunology and Immunobiotechnology, Helmholtz Eye Research Inst in Moscow. From 1995 through 2004, was being a Chairman of the Dept for Clinical Immunology, Moscow Clinical Research Institute (MONIKI) and the Immunologist-in-Chief of the Moscow Regional Ministry of Health. In 1993-1996, was an Executice Secretary-in-Chief of the Editorial Board, Biomedical Science, an international journal published jointly by the USSR Academy of Sciences and the Royal Society of Chemistry, UK.

Abstract:

A new systems approach to diseased states and wellness result in a new branch in the healthcare services, namely, personalized and precision medicine (PPM). To achieve the implementation of PM concept, it is necessary to create a fundamentally new strategy based upon the subclinical recognition of biopredictors of hidden abnormalities long before the disease clinically manifests itself. Each decision-maker values the impact of their decision to use PPM on their own budget and well-being, which may not necessarily be optimal for society as a whole. It would be extremely useful to integrate data harvesting from different databanks for applications such as prediction and personalization of further treatment to thus provide more tailored measures for the patients resulting in improved patient outcomes, reduced adverse events, and more cost effective use of health care resources. A lack of medical guidelines has been identified by the majority of responders as the predominant barrier for adoption, indicating a need for the development of best practices and guidelines to support the implementation of PPM! Implementation of PPM requires a lot before the current model “physician-patient” could be gradually displaced by a new model “medical advisor-healthy person-at-risk”. This is the reason for developing global scientific, clinical, social, and educational projects in the area of PPM to elicit the content of the

new branch.

11:00-11:30

Cheryl Wang

Professor and endocrinologist at Shengli Oilfield Central Hospital., China

Title: Obesity, Inherited homosexuality and LGBT healthcare

Biography:

Cheryl Wang, MD, PhD, earned her MD at Binzhou Medical College, MSc., Endocrinology and metabolism, internal medicine in Shanghai Second Medical University (now Shanghai Jiaotong University), PhD in Science, Endocrinology and metabolism, internal medicine at PLA medical college.

She did internal medicine residency and trained as an Endocrinologist in Donying People’s Hospital, China, did surgery residency at Mount Sinai and Rutgers in the United States.

Abstract:

One major pathogenesis of obesity is the unbalanced hormones, too much stress hormone steroid and/or not enough “anti-obese” happy hormones, endorphin, serotonin, dopamine, and oxytocin, too much estrogen and/or too less testosterone.

Any sexual orientation is normal. Sexual orientation is X-linked and Y-linked traits, inherited by next generations. It is like this, female homosexual XAXA, female bisexual XAXa, female straight XaXa, male bisexual XAYA, male homosexual (manly type) XAYa, male homosexual (girlish type) XaYA, male straight XaYa. The presentation and degree of homosexuality varies in many ways, and at different stages of lifespan, as these hormones and our genetic makeup change. So is fat deposition. More estrogen and/or less testosterone are associated with fat ass. Fat ass may be an easy way to identify stronger homosexuality.

If everybody loves his/her love, it may be better balanced. Yet, the reality is the reality. LGBT healthcare remains far behind. As an unresolved frustration, closeted homosexuality causes a series of health problems, obesity, tobacco/alcohol/substance abuse, and mental/psychiatric disorders. Incautious sexual practice causes sexual transmitted diseases (STDs) like AIDS. Social problems like inequality opportunities among minorities happen often. It brought huge challenge for management. Better acceptance and recognition from learning in a variety way, information merged into clinical visits through smartphone apps and electronic medical record system (ERMS), barrier protection in sexual practice, positive attitude in daily life, team network of physicians, psychiatrists, psychologists, pharmacists, activists, and communities, with loving hearts. Start local, go global, don’t ever shut the door.

10:10-10:40

Nick Kostovic

President Kostovic Acupuncture by bio Electrons Laser, Corp

Title: Cancer cured by bio electron's laser acupuncture

Biography:

Abstract:

its ability to send impulses throughout the body while improving mobility.

Melting and destroying malignant cancer cells in the brain – or any other physical organ – accomplished with no side effects and no harm to healthy cells.

Melting cleanses internal plaque from the vascular system in the brain or any other physical organ. This will prevent strokes, heart attacks, diabetes and many different neurological disorders.

Oral Session 1:

Immunology | ART | Physiology |Oncology | Obstetrics |

12:15-12:35

Kawalya Emmanuel

LABORATORY ADMINISTRATOR WINDSOR LABORATORIES

Title: Accurate And Reliable Fetal Gender Determination Using Primer Design Y-Chromosome Detection Kit

Biography:

Kawalya Emmanuel is a medical professional with extensive knowledge and experience in clinical genomics, bioinformatics, healthcare informatics and economics. He is currently working with Laboratory Administrator Windsor Laboratories in Mengo Hospital Kampala. His main interests lie in the functional sequence data annotation, prioritisation, and interpretation (WGS and WES); multicopy – fetal sex; and integrative/functional medicine.

Abstract:

Aim: To validate a protocol for noninvasive fetal sex determination in maternal plasma and demonstrate its applicability to clinical practice.

Method: Fringe blood from 318 pregnant ladies was gathered from 7 to 12 weeks of incubation. Ongoing PCR was performed for multicopy DYS14 marker arrangement situated inside the TSPY quality by the TaqMan test. An exogenous spiked-in enhancement/identification framework was likewise utilized as a positive control for the extraction procedure. A demonstrative calculation was planned utilizing a blend of these two markers. Fetal sexual orientation controlled by noninvasive pre-birth analysis (NIPD) was contrasted and ultrasound results. The Fisher careful test (two-sided) was utilized to decide affectability and particularity with 95% certainty interims (CIs).

Results: A solitary false-positive outcome was gotten in each of the 318 pregnancies. The general affectability and explicitness were 100% (95% CI 97.7– 100%) and 99.3% (95% CI 96.4– 99.9%), individually. Pregnancy result was gotten in all cases, including 154 male-bearing and 164 female-bearing pregnancies.

Ends: Primer Design Y-chromosome location unit for fetal sex assurance in plasma of early weeks pregnant is exceedingly exact and clinically pertinent if strong revealing criteria are connected

13:45-14:15

Sarwat jahan

PhD, Quaid I azam University, Pakistan

Title: Effects of Furan Exposure on Endocrine Disruption and Reproduction of Sprague Dawley Rats - An F1 Extended One Generation Reproductive Toxicity Study

Biography:

Sarwat Jahan has her expertise in reproduction and passion in improving the health. Her open and related assessment model based on heat induced food reproductive toxicants that disturb the reproductive healthcare. She has constructed this study after years of experience in research, evaluation, teaching and administration both in hospital and education institutions. The current finding is based on F1 Extended one generation reproductive toxicity. The aim of this lab is to provide opportunity for students to specialize in human reproductive biology considering both basic science and clinical aspects of the subject. She has practical experience in major laboratory techniques including histological studies, hormone assays, bio chemical assays, assessment of sperm and the application of molecular biology to reproductive medicines are part of the studies conducted. The lab has active collaboration with Salma and Kafeel Infertility Clinic, Islamabad, Centre of Excellence for bovine genetics Renala Khurd, Okara under control of Pakistan army and Cleveland clinic in America.

Abstract:

Sprague Dawley F0 weaning rats (30/sex/group) were exposed to furan orally at 0, 5,10, 20 and 40 mg/kg/day for ten weeks (males), two weeks (females) and then mated. F0 females were continuously exposed through gestation and lactation of F1 litters. Different parameters in F0 and F1 generation were studied. Results of F0 indicated that the body weight gain decreased during prebreed and gestational period while increased during lactation periods. F0 animals prebreeding exposure resulted in head tilt and foot splay at higher doses. Reproductive indices and gestational length of F0 animals were remained unchanged. Number of live pups at birth were decreased at 20 & 40 mgkg^-1. Weaning pups were distributed into study groups by sex. Survival of F l pups was reduced at 20 & 40 mgkg^-1 only for PND 0 through PND 4. At PND 70, hormonal concentration and histological changes were determined in ovaries and testis. In males, Testosterone and LH levels were decreased while increase in estrogen level of females was seen in 20 or 40 mgkg^-1 groups. Testicular and ovarian weight was reduced in F1 offspring with decreased DSP and disturbed estrous cyclicity in higher doses groups. No histopathological changes were observed in testis and ovaries but in higher doses groups, number of cystic follicles were increased in ovaries. On the basis of above results, it is suggested that furan exposure at 20 or 40 mgkg^-1 exhibit marked changes in extended one generation reproductive toxicity study in F0 (parental stage) and F1 (offspring and pubertal stage) animals.