International conference on
Plant Science and Molecular Biology
Scientific Program
Keynote Session:
Title: Constitutively expressing of the SUC2 gene of yeast encoding of invertase apoplastic localization in transformed potato plants results in multiple biochemical and physiological changes associated with cold resistance
Biography:
Deryabin Alexander got degree of Ph.D. in K.A. Timiryazev Institute of Plant Physiology in 1997 (Visit ours web-site: www.ippras.ru). His major interest is related to cold tolerance of plants and biology of potato tuberization. He has published more than 40 papers in reputed journals, 1 patent, 1 monography and sucsessfully performed 5 research projects funded by Russian Foundation for Basic Research.
Abstract:
The apoplast contains the cell-wall invertase (CWI) (EC 3.2.1.26), which catalyzes the irreversible hydrolysis sucrose (glucose + fructose). This study aim was to investigate the involvement of CWI into tolerance to LT of potato (Solanum tuberosum L., cv. Désirée) plants, which expressed the suc2 gene. WT-potato plants served as the control. The suc2 gene encodes the invertase of Saccharomyces cerevisiae under the control of the tuber-specific patatin B33 promoter of class I with an N-end-connected potato proteinase II inhibitor signal peptide, which provides apoplastic localization of yeast invertase. The suc2 gene presence and its expression were shown using PCR and RT-PCR [1]. Yeast invertase was identified by MALDI-TOF MS analysis. A soluble form of the yeast invertase was present in the apoplast, and it was weakly adsorbed onto the cellular wall [1, 2]. The increased activity of the CWI in transformed plants compared to that in the WT-plants ones promoted more active accumulation of fructose in the apoplast, glucose and sucrose in the leaves and especially, glucose in the roots [3]. The transformed plants were notable for a higher level of sugars in the leaves and, therefore, for a low the rate of lipid peroxidation as compared with the WT-plants. Thus, the potato line with the integrated suc2 gene is a convenient tool to study the role of the CWI during formation resistance to LT and could be found some application in biological engineering.
Title: Alterations in wheat leaf and pollen lipidomes under high temperature stress
Biography:
Sruthi Narayanan earned her bachelor's degree in Agriculture from Kerala Agriculture University, India in 2007. She earned her master's degree in Agronomy in 2011 and Ph.D. in Agronomy in 2015 from Kansas State University. She joined Clemson University as an Assistant Professor of Crop Physiology in 2015. Dr. Narayanan is in the forefront of advancements in crop physiology with cutting edge research on abiotic stress tolerance. Her novel lipidomic approach to characterize plant heat tolerance is widely published and acclaimed. Her research program focuses on understanding tolerance mechanisms of crop plants to abiotic stresses, with an emphasis on drought and heat stresses, at whole-field, whole-plant, cellular, and molecular levels. She is passionate about sustainable agriculture, and has been working on improving resource use efficiencies, maximizing crop yields, and minimizing environmental risk.
Abstract:
High temperature is a major environmental factor that limits wheat productivity. Understanding how wheat (Triticum aestivum L.) plants under high day and night temperature (HT) stresses regulate lipid composition, to maintain stable membranes and generate appropriate signals, is critical to developing climate-resilient wheat varieties. We measured glycerolipids and sterol derivatives under optimum temperature and HT in leaves and pollen of two wheat genotypes, Ventnor (heat tolerant) and Karl 92 (heat susceptible) using electrospray ionization-tandem mass spectrometry. In leaves, levels of 18:3-containing triacylglycerols increased threefold/more under HT, consistent with their possible role in sequestering fatty acids during membrane lipid remodeling. Phospholipids containing odd-numbered or oxidized acyl chains accumulated in leaves under HT. Sterol glycosides and 16:0-acylated sterol glycosides were higher under HT than optimum temperature. Ventnor had lower amounts of phospholipids with oxidized acyl chains under HT and higher amounts of sterol glycosides and 16:0-acylated sterol glycosides than Karl 92. Correlation analysis of lipid levels revealed co-occurring lipids in leaves, which are up-or-down-regulated together through time during HT. Current knowledge of lipid metabolism suggests that the lipids co-occur because they are regulated by the same enzyme(s). Pollen lipidome had a distinct composition than that of leaves. Unlike in leaves, 34:3 and 36:6 species dominated the composition of extraplastidic phospholipids in pollen under optimum and HT conditions. The most heat-responsive lipids in pollen were extraplastidic phospholipids, PC, PE, PI, PA, and PS. PC and PE were negatively correlated. Higher PC:PE at HT indicated possible PE-to-PC conversion, lower PE formation, or increased PE degradation, relative to PC. Taken together, the data demonstrate that lipid compositions of wheat leaves and pollen are altered by HT, in which some lipids are particularly responsive to HT, and similar lipid changes contribute to HT adaptation in both leaves and pollen, though their lipidomes had inherently distinct composition.
Title: The determination of sectional and sub-generic relationships among Vicia L. taxa from Algeria using plant morphology and ISSR markers
Biography:
Sakina BECHKRI is native from Constantine (Algeria). Currently, she is Professor of Genetics at the Faculty of Nature and Life Sciences at Constantine University. She is searcher in the laboratory of “Génétique biochimie et biotechnologies végétales” at Constantine University. His doctoral thesis is entitled: the genus Vicia L. in Algeria. Caracterization of 11 natural taxa: ecogeographic, morphological, biochemical and molecular approaches. She has three scientific publications (consult google scholar citations
Abstract:
The study discusses the genetic diversity and phylogenetic relationships within and between 46 germplasms of Vicia from Algeria. These relations are put in evidence by morphological and molecular markers. For this purpose, 78 plant morphological traits and 4 ISSR markers were used. Diversity of plant morphology has been exploited by UPGMA cluster analysis and principal coordinate analysis, when diversity of ISSR profiles was assessed by cluster analysis using two indices (Dice and Jaccard). Mantel test was used to discuss the correlations between the two. Cluster analysis based on plant morphology revealed two major groups. The results of principal coordinate analysis supported UPGMA clustering. Concerning molecular data, one hundred and thirteen bands were detected, among these bands, 100% were polymorphic. Dice and Jaccard indices showed more or less close patterns in the dendrograms. Mantel test gave a strong correlation between the two indices. Groups generated by plant morphology correspond to the traditional delimitation of the genus. Classification at sectional level also join traditional placement. These findings support suggestions that V. sativa is an assemblage of taxa presenting an overlap in morphological traits. For the other taxa, the separation is sufficient to warrant distinct sectional status for the taxa studied. The primers used could be considered suitable for further variability studies in Vicia genus. However, they have some disadvantages which make their usefulness questionable
Title: Genotypic diversity among Egyptian isolates of Sporisorium scitamineum as revealed by comparative sequence analysis of the internal transcribed spacer and b-east matting type gene
Biography:
Ayman Esh is a Professor and head of plant pathology Dept., at the Sugar Crops Research Institute (SCRI), ARC, Egypt. He also serves as the supervisor of biotechnology and molecular biology laboratories. He also served as the deputy of scientific and research affairs in SCRI and the technical and administrative director of SCRI biotechnology and tissue culture laboratories and greenhouses. Dr. Esh shaped by his first PhD work in sugar beet diseases from Zagazig University and a second PhD in molecular biology and genetic engineering from Sadat City University. He has been involved in many different domestic and international collaborative research projects as a post doctor or a co-PI. He also awarded many international postdoctoral fellowships (USA) and international training programs (USA, France, Italy, Morocco....) in the fields of molecular plant pathology, molecular biology, bioinformatics, tissue culture, ....). Dr. Esh was a co-authored three international books on special topics concerning sugar beet and sugar cane diseases as well as his last chapter on molecular Markers and Phytopatholgy.
Abstract:
A total of 20 Sporisorium scitamineum samples were collected from sugarcane growing areas in Upper Egypt. The genotypic diversity between the twenty S. scitamineum isolates based on ITS1-ITS4 region sequence and b-East matting type sequence were performed. Alignment of the ITS1, 5.8S and ITS2 regions of the rDNA of 20 isolates of S. scitamineum depicting 100% similarities between all the tested isolates except isolate (ADA-1) which has one base difference in the ITS2 region. The obtained two sequences of isolates ADA-1 and HAQ-2 was registered at National Center for Biotechnology Information NCBI Gene bank under the accession numbers JQ912111 and JQ912112 respectively. The combined 18S, ITS1, 5.8S and ITS2 data sets between Egyptian and foreign S. scitamineum sequences obtained from NCBI, grouped the sequences into two groups, the first contained S. scitamineum from Hawaii and the second contained South Africa, India, Egypt, Reunion and China isolates in different levels. The Egyptian S. scitamineum isolate ADA-1 showed a close distance to Reunion isolate while the second Egyptian isolate HAQ-2 was much closer to China isolate. Sequencing data of b-East mating-type gene showed that, all the sequences found identical to S. scitamineum b-East mating-type gene sequence published in NCBI. The b-East gene sequence of isolates ADA-1 was submitted in NCBI under the accession number JQ290342. The multiple sequence alignment of the Egyptian isolates and the published reference sequences of b-East gene from the USA and China of the same organism in the NCBI showed, a single nucleotide polymorphism at the nucleotide number 270 between Egyptian and Chinese isolates
Oral Session 1:
- Plant Science and Soil Science
Chair
Allodane Rajae
University of Abdelmalek Essaadi,Morocco
Title: Overexpression of carnation genes (Dianthus caryophyllus) during the in vitro interaction with Fusarium oxysporum.
Biography:
A biologist with a degree in structural cytogenetics, a specialist in Electronic Microcopy (transmission and lateral scanning), with a Masters in Biochemistry of the host-pathogen interaction in plants and Doctor of Science of plant proteins with activity in the recognition of glycoproteins (lectins). Major areas of performance are Molecular Phytopathology and Phyto-Improvement Assisted by Molecular Markers. Minor areas of performance are Plant Biotechnology, Plant Tissue Culture, Phylogeny and Molecular Taxonomy of Fungi, and Bioinformatics. The academic experience is focused in Molecular Biology and Plant Biotechnology.
Abstract:
The Carnation commercial production in Colombia is the most important item in the flower export business of the country. The principal pathogen that affects the healthy crops is Fusarium oxysporum f.sp. dianthi, which produces the disease known as “basal rot” of difficult handling. The most efficient form of the parasite control is the use of resistant varieties, which are not always available on the commercial characteristics that the market demand. It is not clear the molecular mechanism that produces this resistance to Fusarium in Carnation; in this work, we present the transcriptome of resistant varieties of carnation, produced in a breeding program in our laboratory. The plants were grown under in vitro conditions and nondifferentiated cells were obtained in MS liquid media. The cells were elicited using dual culture techniques with the pathogen Fusarium, the total RNA of carnation cells was obtained by purification in separation columns, and the mRNA was purified using affinity columns; finally, the sequencing was developed by Illumina method. We recognize fourteen different gene groups that could be involve in the response to Fusarium´s presence. In these clusters of genes, we recognize more than 200 putative genes, some of these having a constitutive expression while others are overexpressed during the interaction with the parasite, this overexpression were detected using RT-qPCR technique.
Title: Comparing Logistic Regression and Naïve Bayes in the Identification of maize (Zea mays L.) progeny genotypes
Biography:
Dr SEKA Dagou has completed his Ph.D. from North Dakota State University in 1993. After a period in private entrepreneurship filled with up-and-downs, he returned to academia in 2007 when he started to teach genetics and statistics.
Abstract:
We used two classification methods, Gaussian Naive Bayes and Logistic Regression to predict the genotypes of the offspring of two maize strains, the BLC and the JNE genotypes based on the phenotypic traits of the parents. We determined the prediction performance of the two models with the overall accuracy and the area under the receiver operating curve. The overall accuracy for both models ranged between 72% and 82%. The values of the area under the receiver operating curve were 0.79 or higher for Logistic Regression models, and 0.75 or higher for Gaussian Naïve Bayes models. These statistics indicated that the two models were very effective in predicting the genotypes of the offspring. Furthermore, both models predicted the BLC genotype with higher accuracy than they did the JNE genotype. The BLC genotype appeared more homogeneous and more predictable. A Chi-square test for the homogeneity of the confusion matrices showed that in all cases the two models produced similar prediction results. That finding was in line with the assertion by Mitchell (2010) who theoretically showed that the two models are essentially the same. With logistic regression, each subset of the original data or its corresponding principal components produced exactly the same prediction results.
Oral Session 2:
- Plant Morphology and Metabolism
Title: RAPD Based Assessment of Genetic Diversity in 16 Cultivated accessions of Sorghum in Morocco
Biography:
Rajae Alloudane is a PhD student working in the Biotechnological Valorization of Microorganisms, Genomics and Bioinformatics laboratory at Abdelmalek Essaadi University of Tangier-Tetouan, Morocco. Her thesis project is the study of the genetic diversity of cultivated sorghum by molecular markers
Abstract:
Sorghum (Sorghum bicolor) is one of the most wide spread cultivated grass species in the world. In this study, we analysed the diversity and genetic relationship among 16 accessions of local Moroccan sorghum (Sorghum L Moench bicolor) by using 40 random Amplified Polymorphic DNA (RAPD) markers. 315 alleles were detected ranging between 225 and 2995 bp, 189 (60%) were polymorphic and informative to differentiate the accessions. The number of alleles per locus varied, depending on the accession, from 3 to 11 with an average of 8.66 alleles per locus. The dendrogram, based on UPGMA analysis using similarity of Jaccard coefficient grouped the accessions according to their geographical origin. RAPD markers proved to be a reliable, rapid and practical technique of revealing phylogenetic diversity, which is, should prove valuable for sorghum breeding programs.
Title: High‑level expression of nucleocapsid protein from Canine Distemper Virus in tobacco transplastomic plants
Biography:
- Biography:
Marconi has completed his PhD and postdoc from University of Buenos Aires. The actual position is senior researcher at National Research Council CONICET and team-teache at University Maimonides. She has published more than 25 papers in reputed journals.
Abstract:
Canine Distemper Virus (CDV) produces a highly contagious disease in domestic dogs and other wild animals. Although immunization with attenuated CDV vaccines has been shown to be effective, in recent years there has been an increase in the incidence of CDV infection, including vaccinated dogs. Therefore, a more effective vaccine formulation containing updated immunogens is necessary. The use of plants as biofactories for the expression of antigens is an attractive alternative to produce cost-effective subunits vaccines. In particular, chloroplast transplastomic technology offers stable high-level of recombinant protein expression and transgene containment. In this study, we evaluated the production of the nucleocapsid protein (NP) from an Argentinian CDV strain in transplastomic tobacco plants. Protein expression analysis by Western blot showed the presence of two specific bands in total protein extracts. The expected band of 62 kDa for NP represented approximately 60% of the signal in mature leaves. Furthermore, full-length NP was expressed mainly as an insoluble protein. The concentration of NP in young, mature and old leaves was estimated to be 6.2, 4.4 and 6.7 % of total protein, equivalent to 1.2, 0.7 and 0.4 mg/g fresh weight, respectively. We also evaluated the functionality of the plant-derived NP in a murine model. Oral and subcutaneous immunization with transplastomic extracts elicited an increase of NP-specific serum antibodies compared with control mice. Our results show that NP can be efficiently expressed in transplastomic tobacco plants and maintains its immunogenic properties
Oral Session 3:
- Plant Tissue Culture and Plant Biotechnology
Title: Role of AMF in ameliorating detrimental effect of drought stress on PSI and PSII photochemistry in Wheat plants
Biography:
Dr Sonal Mathur completed her PhD at the age of 34 years from Devi Ahilya University, India and is continuing Postdoctoral Studies as UGC-PDF-SA-II (for Women), from School of Life Science, Devi Ahilya University, India. She was awarded Young Talent Award in 2016 (Russia), and Young Scientist Award in 2014 from Madhya Pradesh Council of Science and Technology, Bhopal, India. She has published 21 papers in reputed journals, with cumulative impact factor of ~ 46.76. She has been serving as a reviewer of few journals.
Abstract:
Water scarcity is becoming a major problem. Drought stress (DS) is amongst one of the most alarming abiotic factors limiting crop productivity. Common symptoms observed for DS are stunted growth, reduced photosynthesis rate, and accelerated leaf senescence. Arbuscular mycorrhizal fungi (AMF) makes symbiotic interactions with ~ 80% land plants. The present work represent role of AMF in protecting photosynthetic apparatus of wheat (Triticum aestivum) from detrimental effect of DS. Wheat (Triticum aestivum; Purna cultivar HI-1544) and AMF culture (mainly comprised of Rhizophagus intraradices; Funneliformis mosseae; F. geosporum) were used for the experiment. DS plants had minimum RWC (Relative water content) for both leaf and soil while AMF plants showed increased RWC indicating that AMF hyphae penetrated deep into the soil and provided moisture to the plants. AMF protected oxygen evolving complex followed by enhanced primary photochemistry of PSII. A down regulation in quantum yields of PSI and PSII, electron transport rate was observed in DS stressed plants. Small PSII antenna size can efficiently restrict PSI photochemistry via donor side limitation which was evident in DS plants (Zivcak et al. 2013). In DS plants, decrease in the quantum yield of PSII and PSI occurred mainly due to an increase in the non regulated energy dissipation and donor-side limitation of PSI. AMF plants had a greater ability for high photosynthetic capacity and prevented the photosynthetic apparatus from being damaged under DS (Mathur et al. 2018). AMF colonization thus helps the plant to maintain integrity and stability of PSI and PSII under drought stress.
Title: Comparative studies of root, shoot and callus cell proliferation from broccoli root tip, shoot tip and leaf cutting slice In Vitro Culture: Antioxidant, Carbodydrate, Pigment and Mineal properties
Biography:
Dr. ABM Sharif Hossain obtained his PhD in Plant Biotechnology (Agriculture) in March 2006, Ehime University, Japan. Dr. Hossain is expertise in Cell and Tissue culture, transgenic cell and plant production, T-DNA, Genetic engineering, hormone, Plant Physiology, renewable energy. He is holding a position as an Associate Professor, Biotechnology Program, Biology Department, Faculty of Science, University of Hail, Hail, KSA. Dr. Hossain has total of 130 Publication including Journal, book, book chapter, book monograph. He has total of 52 Conference abstract and proceeding. He got h-index: 19 and otal citation: 1468. Dr. Hossain supervised PhD: 4 (UM, Malaysia), MSc: 10, Undergraduate : 30 (university of Malaya, Kuala lumpur and Hail University) He is supervision I PhD (National University Malaysia), He has completed 11 research project. He is an Editorial member of 8 Journals. In addition, he is an External Examiner for PhD and MSc Thesis of 5 Universities.
Abstract:
In vitro vegetable culture is an superlative branch of horticultural biotechnology as the broadness of applied plant biology. The study was conducted to investigate the root, callus, shoot and leaf proliferation from the root, shoot tip and leaf cutting in vitro culture using different IBA and BAP concentrations with Ms media. The highest number of root proliferation was found (1.8) in the concentration of IBA 2.0 + BAP 2.0 mg/l combination cultured from root tip. However, the maximum root proliferation was found in the concentration of IBA 2.0 + 1.0 BAP combination cultured from leaf cutting and shoot tip. Positively callus formation was found better in the concentration of BAP 1-3.0 and IBA 1-3.0mg/l combination than other combination of concentrations in the case of root, shoot tip and leaf cutting. Moreover, the leaf proliferation was found better in the concentration of IBA 2-3.0 + 1-3.0mg/l BAP combination cultured from root tip, leaf cutting and shoot tip than other combination of concentrations. The highest callus weight was found in the cultured from shoot and leaf cutting than root cutting in the concentration of IBA 2.0 + 1.0 mg/l BAP combination. Carbohydrate (as inverted sugar and glucose), chlorophyll and nutrient content (K+, NO3-, Ca++ and Na+), total phenol, antioxidant (DPPH), flavonoid and total antioxidant were found in the concentration of IBA 2.0 + 2.0 mg/l BAP combination from broccoli leaf cutting. The present results conclude that it is better to use the combination of the IBA and BAP in the concentration of 1.0-3.0 mg/l to regenerate root, shoot, leaf and callus cell proliferation of broccoli from root tip, shoot tip and leaf cutting.
Keynote Session:
Title: SYNONYMOUS CODON USAGE BIAS IN CHLOROPLAST GENOME OF Vitis vinifera and Two Caucasica Subspecies of Grape Fruit
Biography:
Dr. Farshid Talat is Assistant Professor of Crop genetics and Breeding at Agricultural Research, Education and Extension Organization of Iran. Dr.Talat is deputy director general at West Azerbaijan Agricultural Research Center. He is doing research in the field of crop genetics and biotechnology for 17 years and has been teaching Genetics and Biostatistics in several universities since 1999. He has conducted extensive studies on crop breeding and plant architecture systems in Iran. Dr. Talat served as a coordinator of 40 national projects .He received his B.Sc. degree in Agronomy and Plant Breeding from Urmia University in 1997 and his M.Sc. in Plant Breeding-Biotechnology from University of Tehran in 1999. Dr.Talat received his PhD from Chinese Academy of Agricultural Sciences in Genomics on wild cotton sequencing program. He is the author of 70 national and international published papers
Abstract:
Chloroplast research have significant advantage of genomics and genome sequencing, and a new picture is emerging of how the chloroplast functions and communicates with other cellular compartments. Grape (Vitis vinifera) is a genus of trees in the family Vitaceae. Vitis vinifera species belongs to Eurasian grapes. The chloroplast genome is the most comprehensive genome in plants and has many features for evolution analyses due to the unique molecular structure and single-parent inheritance. Sequence and gene annotation was mainly performed by DOGMA. Map of chloroplast genome structure and gene distribution was carried out using OGDRAW V1.1. Relative synonymous codon usage (RSCU) of different codons in each gene sample was calculated by codonW in Mobyle. An online version of REPuter was used to specify the repeat sequence and location. this research was targeted to study and compare the complete chloroplast genome sequences of Saperavi and Meskhuri mtsvane from caucasia subspecies with common grape (Vitis vinifera) and as well genome structure analysis, gene content, organization and repetitive sequences, codon usage and comparison among genomes. The chloroplast (cp) genome of Vitis vinifera is a circular DNA molecule of 160928 base pair (bp) which is longer than chloroplast genome of Saperavi and Meskhuri Mtsvane cultivars. Large and small unique regions are separated by two inverted repeat regions a, b. In all of three genomes, whole genome contains 131 genes which include 79 protein coding genes, 4 rRNA genes and 30 tRNA genes. In other words, there are totally 113 single-copy genes and 18 double-copy genes located in inverted repeat region (IR) in the three studied genomes. The SSRs of the chloroplast genomes were identified and the results indicated that the chloroplast genomes of Vitis vinifera and Saperavi both have 74 and Meskhuri mtsvane has 73 SSRs. The cpSSRs are important and useful for genetic diversity studies. Low GC content is a significant feature of plastid genomes, which is possibly formed after endosymbiosis by DNA replication and repair.
Title: Plastid Genomics of Wild Grapes (Vitis viniferas subsp. sylvestris) from South Caucasus, Europe and Meditterean Basin
Biography:
The presenting author works on grape genomics during past 10 years. She has numbers of scientific articles devoted to the mentioned field and is participant of different international scientific conferences.
Abstract:
Vitis L. belongs to one of the oldest Vitaceae family of flowering plants. Wild grape Vitis vinifera subsp. sylvestris is a predecessor of cultivated grape and represents the only species of the genus aboriginal to Eurasia. According to many researchers the cultivated grape is believed to have been domesticated around 6000 BC. Resent chemical analyses of ancient organic compounds absorbed into the pottery fabrics from sites in Georgia in the South Caucasus region, dating to the early Neolithic period (ca. 6,000–5,000 BC), provide the earliest biomolecular archaeological evidence for grape wine and viniculture from the Near East, at ca. 6,000–5,800 BC. The discovery of early sixth millennium BC grape wine in this region is crucial to the later history of wine in Europe and the rest of the world. Meanwhile, multiple origins of cultivated grapevine, one in the Near East and another in the Western Mediterranean region are also considered. Over the last years the next-generation plastid DNA genomics has emerged as a powerful and increasingly accessible tool for plant phylogenetics. The main goal of the presented research were: 1. Assessment of genetic diversity of wild grapes from South Caucasus, Europe and Mediterranean basin by using complete chloroplast DNA Illumina sequencing; 2. Annotation of sequenced plastid genomes of wild grape samples from the above mentioned regions. The obtained results are very important for the understanding of wild grape plastid genomes composition and for the study of genetic relationships between wild and cultivated grapes from different geographical locations to explain the molecular bases of grape origin and evolution.
Title: ROLE OF EPIGENETIC IN TREE PHENOTYPIC PLASTICITY IN A CONTEXT OF CLIMATE CHANGES
Biography:
Stéphane Maury is Professor at the University of Orléans (France) INRA, team leader (ARCHE) and has his expertise in plant physiology and epigenomics for teaching and research. He is working for 15 years in the development of epigenomics in crops and trees to better understand the physiology of plant response to abiotic stress and perspectives in plant breeding. He has a long expertise of working with academic and private partners in various projects concerning the role of epigenetic in plants. He is actually the coordinator of a national research project concerning the role of epigenetic in trees adaptation and microevolution (ANR EPITEE).
Abstract:
Global climate changes in progress will impact forest productivity notably through reduced water availability and heat periods. One possibility to adapt is phenotypic plasticity for which epigenetic mechanisms are proposed to be a main source of flexibility. Our objective is to evaluate the potential of DNA methylation to significantly participate to phenotypic plasticity in trees, fixed and perennials organisms with major ecological roles. Over the 10 last years, using an integrative approach with ecophysiological, biochemical, transcriptomics, epigenomics (MeDIP, WGBS, Mobilome) and reverse genetics (RNAi lines) tools, we were able to dissect in the shoot apical meristem (center of the shoot morphogenesis) the response of trees to environmental variations. This work was assessed in distinct experimental set-ups from greenhouse to field plantations as well as during the stress or months post-stress. Our data (recently published and unpublished) showed that Differentially Methylated Regions (DMRs) are associated to active TE and differentially expressed genes with biological functions related to stress response and phytohormone signaling. Altogether, our data proposed that DNA methylation is a source of flexibility associated to phenotypic plasticity in trees opening perspectives for tree breeding. The role of epigenetic mechanisms in tree adaptation and microevolution will be also presented through the results obtained in the frame of the national project EPITREE (ANR-17-CE32-0009-01).
Title: Exploring the synergistic effect and mechanism of Spatholobus Suberectus Dunn extract and doxorubicin in breast MCFâ€7/ADR cells
Biography:
Ms. WEN Yi is a Ph.D student of The University of Hong Kong. She obtained her Master’s degree of Traditional Chinese Medicine in Jiangsu University and Shanghai Institute of Materia Medica. And gained the Bachelor of Pharmacy in Jiangsu University. Ms. WEN Yi was granted the Dragon Culture Traditional Chinese Medicine Scholarship in 2017. And won the Best Paper Award in 17th Tri-University International Joint Seminar & symposium in Thailand. She won the first-class scholarship twice, the second-class scholarship once and also the National Motivational Scholarships during the Bachelor’s degree. Ms. WEN Yi discovered the first novel inhibitor of NAD(P)+-dependent malic enzyme by high-throughput screening and studied the mechanism of inhibitor
Abstract:
Breast cancer is one of the most prevalent cancers in women and the second most common cause of cancer mortality1. Chemotherapy is the major strategy for treating the breast cancer2. The doxorubicin can improve the survive rate of breast cancer patients significantly3. However, the toxicity and drug resistance are the main problem of using the doxorubicin. Grape seed extract (GSE) can reverse the multiple drug resistance by down regulated the NF-kB and YB-1 activity wihich medicatd by MAPK/ERK in A2780/T4. We obtained the most active Spatholobus Suberectus Dunn extract (SSP) by optimizing extraction time, the volume of percolation, the concentration of ethanol. The inhibitory effect of SSP and GSE on breast cancer resistant strains (MCF-7/Adr) is similar, but SSP shows a better synergistic effect than GSE when combining doxorubicin. It suggests that there is another pathway working in the MCF-7/Adr regulate by SSP. Thus, we are exploring the active substance of SSP and the potential mechanism of synergistic effect by cell proliferation, adherent, apoptosis, cell cycle and the amount of protein which related in Estrogen receptor. The aim of the project is to illustrate the mechanism of synergistic effect of SSP and doxorubicin. It helps us understand the Spatholobus Suberectus Dunn, traditional Chinse medicine, in a comprehensive way.
Oral Session 1:
- Plant Pathology and Weed Science
Title: Plant science and molecular biology are the major industries for the development of Health, daily use of life, reduces financial crises, poverty and hunger in the
Biography:
Mr. Muhammad Usman, Former Director General of Agricultural Research System, Government of Pakistan who retired from service after a spotless career of about 35 years with senior level experience on research and development of integrated agricultural production, industries, Plant science and Molecular biology on a sustainable way.
Mr. Usman is basically an agricultural scientist with specialization of agricultural working on the yield and quality of various products.
Mr. Usman is consider as the senior most scientist in the world, always participated in the international conferences as a keynote speech, organizing committee member, moderator of the conferences around the world.
Abstract:
The aim of presentation consist of plant science, molecular biology, health , life , financial crises, poverty and hunger were studied and reported that plant science and molecular biology are the major industries for the development of health, daily use of life, reduce financial crises, poverty and hunger in the world.
Plant science is the study of plant growth, reproduction, evaluation and adaptation as well as the use of plant for food, fiber and ornamental purposes. In simple words, plant science is the study of plant life of a particular region or time. It is the biological characteristics of a particular group of plant. The science of plant is the branch of biology that deals with plant life. In simple words the science of plants means the branch of biology that deals with the plant life. Molecular biology is the branch of biology that deals with the physical and chemical interaction of molecules involved in life function. By using chemical knowledge and techniques, biochemists and molecular biologists can understand and solve biological problems. In the light of the above study, it is proposed that plant science and molecular biology should be commercialized as it is the major industries for the development of health, daily use of life, reduces financial crises, poverty and hunger in the world.
Title: INTERACTIVE EFFECTS OF CADIMIUM STRESS AND PROLINE ON PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS OF FABA BEAN PLANT
Biography:
AMRI ASSIA has completed her PhD at the age of 25 years from Annaba university, Algeria. She is researcher and a teacher at university. She has published 4 articls in reputed journals.
Abstract:
Heavy metal stress is one of the major abiotic stresses that cause environmental pollution in recent decades (Castro et al., 2011). Under stress, Proline accumulated in many plants and acts as a signaling molecule and trigger specific gene expression, which can be essential for plant recovery from stress (Szabados & Savouré, 2009).Therefore, the present experiment was aimed to study the effect of proline on the performance of faba bean under cadmium stress condition. The effect of exogenous application of proline with different concentrations (0, 25 or 50mg / l) on faba bean (Vicia faba L.) plant grown at cadimium levels (0 or 150µM/l.).Under Metal stress condition, all parameters [plant height and root length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, total soluble carbohydrates (TSC), chlorophyll (Chl) a and b, and activity of peroxidase (POD), and catalase (CAT)] of faba beans were strongly depressed, except malondialdehyde (MDA) contents. From these results, proline traitement alleviated the adverse effects of metal stress through increased the photosynthetic pigments, polysaccharides, total carbohydrates, plant height, leaves number, fresh and dry weights of shoot, and seed yield as well as, total soluble sugars, total free amino acids and proline, compared with those of the corresponding cadimium levels, while decreased lipid peroxidation product as malondialdehyde (MDA) and the oxidative enzymes.These results indicate that application of proline was effective, and helped the plant to restore the altered physiological process induced by cadimium stress.
Oral Session 2:
- Agricultural Science
Oral Session 3:
- Plant Genetics and Epigenetics
