Title: Constitutively expressing of the SUC2 gene of yeast encoding of invertase apoplastic localization in transformed potato plants results in multiple biochemical and physiological changes associated with cold resistance

Biography:

Deryabin Alexander got degree of Ph.D. in K.A. Timiryazev Institute of Plant Physiology in 1997 (Visit ours web-site: www.ippras.ru). His major interest is related to cold tolerance of plants and biology of potato tuberization. He has published more than 40 papers in reputed journals, 1 patent, 1 monography and sucsessfully performed 5 research projects funded by Russian Foundation for Basic Research.

Abstract

The apoplast contains the cell-wall invertase (CWI) (EC 3.2.1.26), which catalyzes the irreversible hydrolysis sucrose (glucose + fructose). This study aim was to investigate the involvement of CWI into tolerance to LT of potato (Solanum tuberosum L., cv. Désirée) plants, which expressed the suc2 gene. WT-potato plants served as the control. The suc2 gene encodes the invertase of Saccharomyces cerevisiae under the control of the tuber-specific patatin B33 promoter of class I with an N-end-connected potato proteinase II inhibitor signal peptide, which provides apoplastic localization of yeast invertase. The suc2 gene presence and its expression were shown using PCR and RT-PCR [1]. Yeast invertase was identified by MALDI-TOF MS analysis. A soluble form of the yeast invertase was present in the apoplast, and it was weakly adsorbed onto the cellular wall [1, 2]. The increased activity of the CWI in transformed plants compared to that in the WT-plants ones promoted more active accumulation of fructose in the apoplast, glucose and sucrose in the leaves and especially, glucose in the roots [3]. The transformed plants were notable for a higher level of sugars in the leaves and, therefore, for a low the rate of lipid peroxidation as compared with the WT-plants. Thus, the potato line with the integrated suc2 gene is a convenient tool to study the role of the CWI during formation resistance to LT and could be found some application in biological engineering.