Title: Comparative studies of root, shoot and callus cell proliferation from broccoli root tip, shoot tip and leaf cutting slice In Vitro Culture: Antioxidant, Carbodydrate, Pigment and Mineal properties

Biography:

Dr. ABM Sharif Hossain obtained his PhD in Plant Biotechnology (Agriculture) in March 2006, Ehime University, Japan. Dr. Hossain is expertise in Cell and Tissue culture, transgenic cell and plant production, T-DNA, Genetic engineering, hormone, Plant Physiology, renewable energy. He is holding a position as an Associate Professor, Biotechnology Program, Biology Department, Faculty of Science, University of Hail, Hail, KSA. Dr. Hossain has total of 130 Publication including Journal, book, book chapter, book monograph.  He has total of 52 Conference abstract and proceeding. He got h-index: 19  and otal citation: 1468. Dr. Hossain supervised PhD: 4 (UM, Malaysia), MSc: 10, Undergraduate : 30 (university of Malaya, Kuala lumpur and Hail University) He is supervision I PhD (National University Malaysia), He has completed 11 research project. He is an Editorial member of 8 Journals. In addition, he is an External Examiner for PhD and MSc Thesis of 5 Universities.

Abstract

In vitro vegetable culture is an superlative branch of horticultural biotechnology as the broadness of applied plant biology. The study was conducted to investigate the root, callus, shoot and leaf proliferation from the root, shoot tip and leaf cutting in vitro culture using different IBA and BAP concentrations with Ms media. The highest number of root proliferation was found (1.8) in the concentration of IBA 2.0 + BAP 2.0 mg/l combination cultured from root tip. However, the maximum root proliferation was found in the concentration of IBA 2.0 + 1.0 BAP combination cultured from leaf cutting and shoot tip. Positively callus formation was found better in the concentration of BAP 1-3.0 and IBA 1-3.0mg/l combination than other combination of concentrations in the case of root, shoot tip and leaf cutting. Moreover, the leaf proliferation was found better in the concentration of IBA 2-3.0 + 1-3.0mg/l BAP combination cultured from root tip, leaf cutting and shoot tip than other combination of concentrations. The highest callus weight was found in the cultured from shoot and leaf cutting than root cutting in the concentration of IBA 2.0 + 1.0 mg/l BAP combination. Carbohydrate (as inverted sugar and glucose), chlorophyll and nutrient content (K+, NO3-, Ca++  and Na+), total phenol, antioxidant (DPPH), flavonoid and  total antioxidant  were found in the concentration of IBA 2.0 + 2.0 mg/l BAP combination from broccoli leaf cutting. The present results conclude that it is better to use the combination of the IBA and BAP in the concentration of 1.0-3.0 mg/l to regenerate root, shoot, leaf and callus cell proliferation of broccoli from root tip, shoot tip and leaf cutting.