Title: Plant secondary metabolites: A good source of herbal antibiotics

Biography:

Dr Priti Mathur   is working in Amity Institute of Biotechnology, Amity University Uttar Pradesh, and Lucknow as Senior faculty. Dr Priti Mathur has proved first time in world that antibiotics like compounds can be isolated from plant source. This is evident by her recent research papers published in national and international journal and patent “herbal antibiotic formulation” submitted to patent office, Government of India. Dr Priti Mathur is working on “Isolation and characterization of antibacterial compounds from wildly growing plants” with her students with   collaboration with Ram Manohar Lohia Institute of Medical Sciences and Central drug research institute, Lucknow. Dr Mathur completed her Post graduation Patna University, Bihar India. Dr Priti Mathur has done PhD from CSIR organisation- Centre Institute of Medicinal and Aromatic Plants and Post doctorate from ICAR organization -Indian Institute of Sugarcane research. She has filed two patents and published many research papers in reputed national/ international journal and presented her work in various national/international conferences. She has been awarded by fellowships and award for her research work. 

Abstract

Our planet is full of natural resources. Nature has given us plants and its product to live the life comfortable and healthy. It has been predicted that there are 30,0000 to 50,0000 different plant species in the world with biologically & chemically diverse groups. According to one report approximately 20,0000 secondary metabolites are present as specialized compounds. The existence of this biologically active substance within the plant tissue is responsible for its therapeutic value & includes alkaloids, glycosides, flavonoids, tannins, resins, essential oils, gums, etc. These phytochemicals provide a frequent motivation of therapeutic agent with reduced toxicity, broad spectrum of activity & good pharmacokinetics to be used clinically deprived of any chemical alteration. There is urgent need of effective antibiotics to overcome global problem of antibiotic resistance in clinically relevant bacterial populations. Plants are excellent source of antibacterial compounds. We have we have isolated and characterize,a plant steroid  Dinoxin B withanolide as a potential antibacterial compound from Datura inoxia against Staphylococcus aureus.our research work done by both wet and dry lab techniques worked on mechanistic study to strengthen Dinoxin B withanolide as potential herbal antibiotic. We have compared ultra-structure of treated and untreated Staphylococcus aureus by Dinoxin B withanolide, using transmission and scanning electron microscopy. The bactericidal effect of compound was also analysed by studying bacterial DNA and protein using electrophoresis techniques. Furthermore, cytotoxicity of the compound was evaluated on J744 cell lines using MTT assay. Moreover, shelf life of the compound was also studied at different time intervals. To identify target molecule docking studies using Maestro 12.5 Schrodinger software, performed. Pharmacological properties of Dinoxin B withanolide also analyzed using same software. Our electron microscopy studies showed that compound was found to damage bacterial cell wall and membrane. It also causes complete degradation of DNA and protein, which hampered bacterial growth and finally resulted in the bacterial death. The compound was also found non-toxic to mammalian cells, suggesting that it only inhibits necessary biological processes in the bacteria without affecting the host cells. These findings confirm the therapeutic applicability of Dinoxin B withanolide, due to its safety and efficacy also confirm our published   previous bioassay studies. Penicillin Binding Proteins (PBPs) identified as target molecules, which is present on cell wall. Pharmacological results confirm that Dinoxin B withanolide could be used as herbal antibiotics and will proved as revolutionary in pharmaceutical industries. Further, research is needed to explore the inhibitory action of Dinoxin B Withanolide on animal models followed by pharmaceutical formulation and clinical trials for the welfare of mankind.