Title: Evaluation of anti-proliferative and apoptotic effect of resveratrol on AGS cell line of gastric adenocarcinoma

Biography:

Researcher of Institute Pasteur,Amol Branch 2000-2012
Assistant Prof of Pharmacology MUMS 2012-now
6 months scholarship QIMR,Brisbane Australia 2009
3 months grant, trainee ship, Armand Frappier Institute,Montreal Canada
Editorial board of sciafrijournal
Editorial board of International Journal of Interdisciplinary and Multidisciplinary Studies (IJIMS)
Published Articles :46
Articles in ISI or PubMed:15
Research projects:30
PharmD or Bsc Thesis supervisor:30
Published books:2
Iranian Pharmacy council member
Iranian Medical council member
Australian Society of Medical research (ASMR) member
Fields of researches:
-Cancer Pharmacology
-Neuro-Pharmacology
-herbal medicine researches and diabetes

Abstract

Resveratrol is a phenolic natural product which is found in red grapes also in Japanese knotweed root(polygonum cuspidatum) .It is also found in Gnetum cleistostachyum.In recent years, many researches were about anti-inflammatory and anti carcinogenic effect of resveratrol especially in  breast, colon and esophageal cancer, but a few researches were about its' role in gastric adenocarcinoma.This research has been done about role of resveratrol in an In Vitro model. For proliferation study we used MTT assay procedure and for apoptosis ,Anexin-propidium iodide  flowcytometery method has been done .Also we have used AGS cell ,The cells  incubated with Resveratrol and Cisplatin ( as Positive Control)  for 24 and 48 hr. in different concentrations ,IC50 has been determined and compared with control. Our results showed that reveratrol in IC50 concentration( 40.6 µM) has significant antiprolifrative  effect in AGS cells  compared with control which was in parallel with cisplatin .Also at IC50 concentration  6.75% apoptosis and 10.8% necrosis has been observed after 24 hr.incubation. According to our results resveratrol has significant antiproliferative and apoptotic effect as in vitro model of gastric adenocarcinoma and can be suggested for co-treatment in future chemotherapy of gastric malignancies