Title: Development of Biosensor for Surra (Trypanosoma evansi) based on protein approach using differential pulse voltammetry method

Biography:

April Hari Wardhana currently works at the Department of Parasitology, Indonesian Agency for Agricultural Research and Development. April does research in Biotechnology, Entomology and Parasitology. Their current project is 'Chrysomyia bezziana, Black Soldier Fly, Vectors of Trypanosoma evansi, Trypanosoma evansi, and Protozoan Gastrointestinalis (Giardia, Entamoeba. Eimria and Cryptosporidium)'.

Abstract

Trypanosoma evansi remains a major cause of Trypanosomiasis in Indonesia, called Surra. However, the lack of accessibility and affordability for diagnostic tests (Card Agglutination Test for Trypanosomiasis/T. evansi – CATT/T. evansi; ELISA and PCR) leads to unsuccessful control of Surra in the field. The study aims to investigate the performance of protein-based biosensor of surra (Trypanosoma evansi) for distinguishing positive and negative sera. Herein, protein was obtained from the T. evansi propagation. This protein was immobilized by carbodiimide reaction on the surface of carbon screen printed electrode as the lowest interaction material with serum/antibody (% I < 90%). The sensor method used in this present study was differential pulse voltammetry (DPV).