Title: Protective role of ascorbic acid on subacute toxicity in rabbits treated with ivermectin: Biochemical and histopathological investigations

Biography:

Hassina Khaldoun Oularbi working as a Professor at Department of Biology in University of Blida 1, Algeria. Her research interest in Pharmaceutical, Medicinal and Organic Chemistry.

Abstract

Ivermectin (IVM) is a lipophilic anthelmintic drugs widely used for the control of internal and external parasites in both clinical and veterinary medicine. The present experiment pertains to the protective role of ascorbic acid (AA) against biochemical and histopathological toxicity induced by subacute exposure to ivermectin in male young rabbits «Oryctolagus cuniculus». Twenty rabbits were divided into four groups of five. Control group and ivermectin treated groups subcutaneously with a high dose (2 mg kg⁻¹ of body weight) of ivermectin (3 times/week) for three consecutive weeks, alone (IVM-group); or orally co-treated with ascorbic acid by gavage (group-IVM+AAg) (20 mg/kg/mL) or AA food supplemented (group-IVM+AAf) (200 mg/kg/food) respectively, with a mean interval of 24 h after IVM injection. During the experiment, ivermectin alone caused signs of toxicity (hair loss and diarrhea) and disruption of the kidney and liver biochemical parameters and lipid profile with statistically significant increase (p <0.05) in blood glucose and levels of liver enzymes (ALAT, ASAT and γ-GT) at day14 and day21. However, the co-administration of the ascorbic acid has moderately corrected and improved these biochemical parameters. Light microscopic observations revealed variable signs of brain, renal and hepatic toxicity in the IVM group alone. However, AA treatment showed significant amelioration when coupled orally with IVM. These findings suggested that AA has beneficial influences in neutralizing the sub-acute toxic effects of IVM in the biochemical parameters and histology of liver, kidney and brain of rabbits.