Title: Extraction and determination of Ertapenem antibiotic in forensic matrices by solid phase extraction followed by LC-MS

Biography:

Abstract

Antibiotics are pharmaceuticals which are used to cease and cure bacterial infections. Due to their rapid consumption, it leads to addiction following antibiotic abuse. Cases have been reported for antibiotic overdosage. Since not having certain protocols to detect such cases we have developed a method to detect such antibiotics from postmortem samples. A solid‐phase extraction based on two organic solvents followed by isocratic liquid chromatography and mass spectroscopy with 242nm UV‐vis detection was achieved for determination of ertapenem antibiotic, in forensic samples. Methanol was selected as the supported liquid membrane. The elution was done by formic acid. Chromatographic separation of the drug was achieved on a Hypersil ODS C-18 150mm X 4.6mm, 5μm column using a mobile phase consisting of a binary mixture of Phosphate buffer (4.5590gm of Potassium dehydrogenate orthophosphate in 1.0 liter Water and pH was adjusted to 7.5) and Methanol in the ratio of 60:40v/v. To evaluate the capability of the proposed method in the analysis of biological samples, three different forensic samples were analyzed under the optimal conditions. The relative recoveries of the ertapenem in three different forensic samples were above the range of 70%. Thus, the developed Liquid Chromatographic method offers symmetric peak shape, good resolution and reasonable retention time for the drug. Linearity, accuracy and precision were found to be acceptable over the concentration range of 10-40microgram/ml for ertapenem.