The use of mesenchymal and progenator stem cells for wound care and stimulation have been proven to accelerate  wound healing through the secreted growth factors obtained within the cells. Bone Marrow (BM-MSC’s) and Adipose-derived (ADSC’s) have been shown to accelerate dermal fibroblasts  secreted growth hormones. BM-MSC’s are conditioned to release proteins like IGF-1, keratinocyte growth and stromal derived factors(-1), as well as EGF, angiopoietin-1, alpha and beta proteins and more. Both BM-MSC’s and ADSC’s are being used to created concentrated mediums and scaffolding to advance wound healing. Clinical evidence has been shown to report the use of ADSC’s to be a substantial substitute in the absence of BM-MSC’s. Complexity of the wound will disrupt the synergistic response that this therapy elicits from the injury. But, research and clinical based evidence surrounding progenitor allogenic, autologous and xenogeneic regenerative therapies have showed promising research in healing chronic injuries and adhesion formations. In current medical practice, it is recommended that gene delivery be administered through liposomal vectors and high pressure administration. BM-MSC’s and ADSC’s have been proven to be beneficial through the use of allogenic donor stem cells via intradermal administration into full-thickness cutaneous wounds and accompanying localized inflammation. By administering MSC’s to treat wounds; collagen synthesis, angiogenesis and cellular proliferation occur more rapidly than with a wound attended to without the use of regenerative medicine. Platelet rich plasma (PRP) has also be utilized heavily in wound healing management. Popularity of PRP in small animal medicine has been gaining grounds in the past decade due to the platelets ability to replicate the normal morphology of the surrounding tissues with similar characteristics. The mechanisms of which BM-MSC’s, ADSC’s and PRP are still wildly unknown when we consider the imbalance that’s produced when the sample cells are missing or depleted of certain chemokines and cytokines.

The employment of single-use technologies is a bright example of the progress in modern medicine. The Age of AIDS and hepatitis C dictates its terms on the treatment strategy and tactics in different surgery directions. The idea of the single-use principle application of a sophisticated high-tech instrument on a stage of surgical treatment of injuries, generally, seemed to be a “crazy idea”, according to many experts. The path to the practical implementation of the project called “Electrodermatome – ДК- 717” was quite long and complex. The idea of a single dermatome was patented in 2007 (patent UA32875). The first prototype of the invention was improved and modified after trials. The author of the invention faced with the complex task of changing the metal elements of the cutting part of the instrument into polymeric ones and developing an absolutely new instrument design. Because only on this condition mass production of single -use cutting heads becomes possible. For this purpose, multicomponent high-precision mold (weighing 200kg), used for the production of polymeric parts of the mechanism, had been developed for 3 years. Thus, the details are formed in the die-casting mold through the use of casting machine, called thermoplastautomat. Tool buildings are held in the specialized production laboratory (in Ternopil). Yet, the system of analog mechanisms is used, and the calibration of instruments is held under guidance of binocular stereomicroscope. A single-use head consists of polycarbonate parts and metal elements that eliminates complex technological stages, milling of products, and, consequently, ensures tool cost reduction in 10 times. The cutting head of the dermatome is accurately calibrated and provides high quality of the tool, and once it is a single- use one, it helps to avoid the bit wear. The design of the electric dermatome disc (a tool used for surgical cure of burn patients) proved to be interesting. The innovation of the tool lies in invention of a single-use blade of annular (ring) shape. High precision metal laser-beam cutting technology, which eliminates stages of hard and costly handling, is used in the blades manufacturing. It should be noted, that in this time of dermatome, reusable blades were used. After sterilization blades quickly wore out and then the tools broke down. New design solutions are proposed towards skin transforator and linear dermatome, they are equipped with reusable cutting heads. Dynamic drive system that drives the cutting mechanism of different models of dermatomes also has innovative features. The system is equipped with an electronic charging indication display. Long operating cycle and high ergonomics of the tool are provided by a modern lithium-polymer battery. The operation, charge indication and charging process are controlled by electronic unit. The drive system is tight and may be sterilized through gas sterilization, if necessary. The reliability of the instrument provides modern commutatorless motor with high power and low energy consumption.

In day to day practice, the wounds particularly complex ones like - infected and necrotising; with exposed tendons, bones, implants; chronic & non-healing ulcers- pressure sore present a substantial clinical challenge. They need intense management with multiple surgical intervention, antimicrobial /antibiotics, analgesics, and local care; requiring huge resources, skilled manpower and experts. Despite best of care the results are unpredictable and may be associated with severe morbidity, at times. This study is undertaken to develop a comprehensive solution based on regenerative medicine for such enormous health problem.

The Intervention - “STARS therapy (Sandeep’s technique for assisted regeneration of skin)” with Autologous Platelet Rich Plasma (PRP), has been developed and clinically piloted on 268 wounds. It is based on the local infiltration of freshly prepared autoogous PRP. It is the mainstay and mono therapy for the wound management. The results reveal a complete healing with control of infection, filling up of defects and assisted regeneration of skin.

For the first time, reversal and revival of grossly necrosed and devitalised tissues such as tendons, muscles and bones has been clinically achieved. The history of Wound management over 4000 years have been till now have been consisting of mostly local applications & Dressings, Drugs and Devices such as negative suctions etc.

With development of regenerative medicine, we are looking into an exciting option and possibilities. The PRP is one of the simplest Regenerative medicine products having huge possibility of reproducibility amongst all health settings across the globe. A comprehensive effective mono-therapy solution based on the PRP only, will have a huge impact in improving wound care   across the globe. This “STARS therapy” is a simple safe and effective, such solution and a complete Game Changer benefiting hugely scores of patients suffering from such conditions and its morbidity.

The use of stem cell therapeutics has become one of the largest areas of scientific research around the globe. It was first introduced into equine practice in 1995 with the injection of bone marrow derived cells into tendon injuries. The use of stem cell in canine bone marrow transplants date back as early as the 1960’s. Since, it has found great interest in its use in small animals and humans for many different medical conditions, in particular osteoarthritis. It is estimated that 10 to 12 million dogs in the US are affected with osteoarthritis, and it is the most common cause of chronic pain. Stem cells are unspecialized cells capable of self-renewal and differentiation into a specialized cell, and perhaps any type of tissue in the body that is needed. Another term for regenerative medicine is Prolotherapy. Prolotherapy is a regenerative injection treatment used to stimulate the healing mechanism to repair damaged or injured areas in the spine and joints. This term may be inclusive of both stem cell therapy and platelet rich plasma. Both treatment modalities can be used in conjunction to serve as a transport to the intended treatment area. Regenerative medicine therapies should only be implemented or recommended following a thorough examination with a Veterinarian. Diagnostic imaging and further treatment options should be discussed prior to starting prolotherapy as it is known to be contraindicated in the presence of cancer, infection, coagulopathies, NSAID’s and steroids. The patients are monitored closely before, during and after initiating treatment for any adverse effects. By routinely tracking our patients closely with examinations, surveys and all otherwise client communications; we are able to adjust their therapies as needed and determine the patients progress.