Dr. Sudha Bansode is a Professor in Zoology at Shankarrao Mohite College, Akluj, and Maharashtra State, India. Recently she has completed her Post-Doctoral Studies at University of California, Riverside, USA. She is a active researcher & passionate teacher in India. Still she has been published above 25 research papers in International Journals & she is interested on Bone Research. Also she has honour of Distinguished Editorial Board Member of several International Journals. She is a own author of Textbook Histological Techniques & Outlines of Physiology. And now she is working on another own reference book Rhythms in Freshwater Crustaceans. She is a University recognized research guide for Ph. D. students in India.
She was a invited Indian Speaker of OXFORD SYMPOSIUM on27-29 August, 2014 at Balliol College, Oxford, United Kingdom & CELL SIGNALING & CANCER THERAPY – International Conference at Double Tree, Hilton Chicago on 27-28 September 2017. She was academic visitor of Bangkok- Thailand, Colombo-Sri Lanka, Daira-Daira-Dubai-UAE. Her recent intellectual Interaction is with many International Professional groups.
Abstract
Abstract:
Cell culture has become an indispensable technology in many branches of the life science. It provides the basis for studying the regulation of cell proliferation, differentiation & product form at ion in carefully controlled conditions with processes & analytical tools which are scalable from the level of single cell to in excess of 10kg wet weight of cells. The cell culture has provided the entire human genome, and to dissect the pathway soft intracellular & intercellular signalling which ultimately regulates gene expression. As roller bottle culture is considered the first scale up step for choragic dependent cells from stationary flasks or bottles (Langdonetal2004). This achieved by using all internal surfaces for cell growth, rather than just the bottom of the bottle. The added advantages are that a smaller volume of medium & thus a higher product titre can be achieved the cells are more efficiently oxygenated due to alternative exposure to medium and gas phase &dynamic systems usually generate higher unit cell densities than stationary systems.
Keywords: Cell culture/Animal tissue culture / stem cell research