Title: Fermentation strategy for enhancing phytase production under D-mannitol co-induction in pichia pastoris

Biography:

Dr. Ndayambaje Jean Bernard is a lecturer in the University of Rwanda. He holds PhD. of Technology in Industrial Biotechnology from Anna University and MSc. in Chemical engineering from XiamenUniversity. The first intention of his research orientation is in cloning technology, optimization andexpression at small scale and large scale fermentation. Furthermore I’m International Membership in the Technical committee/RSB of Quality assurance of Pharmaceuticals and Quality Management System and also Associate editor in the journal of Genetic Engineering and Biotechnology and African Journal of Biotechnology.

Abstract

The addition of D-mannitol with methanol as a co-substrate at the induction phase during fermentation by Pichia pastoris (Mut+) is a new beneficial technology for recombinant protein production. The major challenges as heat generation and high oxygen demand are increasing during induction phases with methanol at large scale and this causes high oxygen demand during cultivation. One possible way to reduce the oxygen demand for getting more protein productivity is the addition of D-mannitol along with methanol during induction time. The promoter of alcohol oxidase I (AOX1) gene in P. pastoris is exclusively matched for the controlled expression of foreign genes and the high levels of foreign proteins can be expressed, even if they are lethal to the cell. The co-feeding strategy was optimized to produce phytase activity of 10280 U/ml compare to methanol fed alone and which it could be used as food additives for non ruminant animals. A deep understanding of the regulation of AOX1 promoter, the physiology of the cells and which are being used to govern protein production during methanol/D-mannitol strategy is still not developed at large.