International Microfluidics Webinar
Scientific Program
Keynote Session:
Title: Simulation and 3D printing manufacturing of microfluidics for continuous particle separation with applications in monitoring air pollution
Biography:
Hamid Sadabadi is an entrepreneur and researcher in the field of Microfluidics, Lab-on-a-chip and sensors/biosensors. He has completed his PhD in microfluidic from Concordia University in Montreal. He is recipient of 8 prestigious awards/scholarships inducing Quebec Doctoral Merit Scholarship and University of Calgary Eyes High Postdoctoral Fellowship where he did his postdoc researches. He is the currently CTO of Wireless Fluidics, a sensing technology development start-up. He is also currently invited to be an assistant professor and researcher at American University of Middle East. He has published more than 12 US patents, book chapters, and more than 16 papers in reputed journals.
Abstract:
An inertia microfluidic chip is designed to separate small particles suspended in a liquid. ANSYS fluent was further used to simulate the separation efficiently. Assuming the 3D printing resolution, the minimum printable feature size was assumed to be 850um. For results validation, the chip was made by a 3D printer and the separation was tested in the chip. Two particle sizes (50um and 5um) were used for testing. The particles were mixing first with ethanol and then diluted in water in order to create a suspension. A syringe pump then used to inject the flow into the chip. The results show good separation of particles with the selected design and in accordance with simulation results.
This results showing proof of principles of using day-today 3D printing tools for fabrication of microfluidics which can eliminates the needs for advanced manufacturing steps like photo-lithography for microfabrication of chips for particle separation. One of the main application of this technology is to enable separation of big dusts and air prolusions (which are in the same size category) for air quality monitoring in the GCC countries which is a big challenge. The next phase of this work is to integrate this chip with a sensing method (RF sensing) for continuous monitoring/detection.
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Title: Micro-flow analysis with monolithic columns
Biography:
Petr Solich is the Vice-dean for strategic development and European funds, Professor, Head of the Department of Analytical Chemistry, Group Leader of Analytical Chemistry Group (ACG). He Guarrantee of OPVVV project Establishment of Specialized Team for Advanced Research on Separation Science” STARSS, Guarrantee of OPVVV project „Efficiency and Safety improvement of Current Drugs and Nutraceuticals: advanced methods – new challenge“ - EFSA-CDN.
Abstract:
Miniaturization in flow analysis can be done by several ways, one of them is Sequential Injection Chromatography (SIC), which use monolithic columns for separation processes and presently is already becoming well-established analytical technique.
Monolithic materials proved their role both as sorbents for solid phase extraction and chromatographic separation. These methods profit from large active surface (mesopores) and highly porous structure (macropores) of the monoliths. Although available commercially, significant benefit arises from ease of their preparation in laboratory. Numerous approaches can be used for preparation of monoliths leading to materials varying in active surface, porosity, chemistries, polymer properties, and size. This flexibility results in extraction and separation sorbents including formats such as pipette tip for solid phase extraction (SPE), micro-column SPE, well-plate SPE, and HPLC analytical and capillary columns that are finding applications in manual, semi-automated, and on-line methods. Typical target samples include complex environmental and biological matrixes, as well as all kinds of inorganic and organic analytes including biomolecules. A broad range of micro-flow analysis methods have already been developed using monoliths .
Fundaments, overview, trends, and perspectives of monoliths in micro-flow analysis will be discussed. An overview of several recent applications of the use of monolithic columns in micro-flow techniques will also be pointed out .
Oral Session 1:
- Oral Session 1
Chair
Bruce McCord
Florida International University, USA
Title: A microfluidic-based sensor with a built-in probe for conformal mechanical measurements of different anatomical sites at the exterior surface of human Pectus Carinatum (PC) costal cartilage
Biography:
Jiayue Shen received her Ph.D. degree from Old Dominion University, Department of Aerospace and Mechanical Engineering in 2018. During the same year, she joined the College of Engineering, SUNY Polytechnic Institute as an Assistant Professor. Her research interests focus on flexible electrodes, microfluidic-based sensors, soft robots and related applications. She has published more than 16 journal papers and conference proceedings and has been serving as reviewers for various journals and international conferences. Additionally, she has been serving as a conference committee member of 2019 2nd International Conference on Smart Sensing and Intelligent System as well.
Abstract:
This study aims to measure the viscoelastic properties of different anatomical sites at the exterior surface of human Pectus Carinatum (PC) costal cartilage (CC) tissues via a microfluidic-based sensor, and determine whether the viscoelastic properties show a link with the anatomic sites and the cartilage length. Five CC segments from the 7th ~10th ribs are obtained from a 15yr-old PC patient. Using a testing protocol of multiple indentation-relaxation steps, four anatomical sites: anterior/posterior surfaces and superior/inferior borders are measured at locations of 6mm apart along the length of each CC segment. The instant indentation modulus and normalized relaxation amount are derived from the recorded viscoelastic response to quantify the elasticity and viscosity at each measured site of the CC segments, respectively. These CC segments are found to be stiffer and less viscous than healthy porcine CC. The normalized relaxation amount reveals a decreasing trend with the indentation depth in the range of 80µm~240µm, but becomes stabilized in the indentation range of 240µm~480µm for all the CC segments. Overall, the anterior surface is stiffer than the posterior surface, which is opposite to porcine CC and is possibly due to different gravitational forces acting on them. For all the CC segments, the instant indentation modulus and normalized relaxation amount both reveal a considerable, random variation among the four anatomical sites at the same location. However, the average instant indentation modulus and average normalized relaxation amount from the four sites at the same location both do not change much along the cartilage length, indicating that the rest anatomical sites might adjust to accommodate the change at one anatomical site. Only one of the segments show a decreasing trend of instant indentation modulus along the cartilage length and exhibits mild variation in elasticity and viscosity among the four sites and along the cartilage length.
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Title: Review of microfluidics device for drug delivery and formulation screening
Biography:
Romain Delamare has completed his PhD on nanostructured materials in 2003 from Orleans University and Postdoctoral Studies from Institute of nanosciences (IM2NP) , Marseille University, France. He was the Director of Winfab (Louvain-la-Neuve, Belgium) , a micro-nano fabrication platform for 8 years. He is now the head scientist of the research department of Medincell. He has published more than 30 papers in reputed journals and has been serving as an conference organiser of repute in the field of nanofabrication .
Abstract:
Microfluidic devices present many advantages for the development of efficient drugs as they offer rapid techniques for direct drug screening. They not only optimize resource management, but also enable massive parallelization for tests with significant economies of scale. The precise control of experimental conditions and the very low volumes involved in microfluidics solutions match the requirements of 2D and 3D cell cultures as well as organs on a chip, which is key to narrowing the bridge between in vitro and in vivo environments. Existing preclinical models are still inefficient for predicting clinical outcomes and microfluidic devices offer a more rapid and cost-effective alternative. In this review, we will highllight microfluidics microfabrication methods and knowhow exploited in the field of drug delivery. And then, we will discuss the interest of microfluidic devices for use at point of care as well as organ on a chip models as smart, sensitive, and reproducible platforms for the drug testing under bio like conditions.
Title: Will update soon
Biography:
Reza Abdolvand is an associate professor and the director of the Dynamic Microsystems Lab in the Department of Electrical Engineering and Computer Science at the University of Central Florida, where he joined in January of 2014. He received his B.S. and M.S. degrees in electrical engineering from Sharif University of Technology, Tehran, Iran, in 1999 and 2001, respectively, and the Ph.D. degree from the School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA in 2008. His research interests lie in the area of micro/nanoelectromechanical systems with a special focus on design, fabrication, and characterization of micro-resonators with applications in radiofrequency circuits and resonant sensors including bio-sensors.
Abstract:
Will update soon.
Title: Microfluidics for cancer research
Biography:
Valerie Taly is the research Director in the The French National Center for Scientific Research, France. Her group (Translational Research and Microfluidics, TRAM) aims at developping and using microfluidic systems to perform experiments that are out of the possibilities of existing technologies. We develop new tools, procedures and strategies for Cancer Research with three major focuses:
(i) Creating new generations of devices for the non-invasive detection of Cancer biomarkers with applications in personalized medicine, cancer recurrence detection and cancer diagnostics.
(ii) Highlighting of new Cancer biomarkers.
(iii) Developing whole platforms for toxicological analysis of chemical and drugs.
Abstract:
Will update soon.
Oral Session 2:
- Oral Session 2
Chair
Romain Delamare
Medincell SA, France
Title: Inkjet microfluidic technology for printing and life science applications
Biography:
Alexander Govyadinov has over 35 years of experience in various sensing platform development in academic and R&D industrial environments, and recent 18 years works for Hewlett-Packard printing, and after the Company split for HP Inc. In Advance Technology and Product Development Organization developing novel sensing and microfluidic solutions for inkjet and other applications. He developed Light Scattering Drop Detection concept implemented in HP first page wide array printers Office jet prox series. Last decade he led development of novel concepts of microfluidic architectures enabling HP advanced inks and microfluidic components and systems for life science applications. He is co-author of multiple scientific publications and over 100 US Patents and patent applications.
Abstract:
Recently, there has been a lot of interest in microfluidic lab-on-a chip applications for life sciences, forensic, point-of-care, molecular-diagnostic, other in-vitro-diagnostic, organs- on-a-chip, environmental and other applications. Various scientific and commercial organizations explore different material sets and operational principles to forge microfluidic devices. Simultaneously, the inkjet industry is repurposing its well-developed material base and manufacturing processes for large scale fabrication of complex microfluidic systems for precision dispense, droplet manipulation and other applications. The presentation describes our recent progress in the development of a low-cost microfluidic platform utilizing the materials and processes of the commercial thermal inkjet business. The well-established microfluidic components and jetting elements are being repurposed for pumping, mixing, valving, fluid transport, sensing and other critical functions of complex integrated microfluidic systems. This presentation describes the operating principles of microfluidic elements, gives examples of their integration in functional devices and discusses the potential of the inkjet technology to deliver a broad range of microfluidic applications and lab-on-a-chip diagnostic devices.
Title: Will update soon
Biography:
Kari Ullakko is Professor (tenured) and Head of the Material Physics Laboratory at LUT University. He has worked with several universities and companies in various countries and has led a number of international research teams and projects (Finland, EU and USA) and gained research funding over 23 Million euros. His discovery of the magnetic shape memory effect has created a new field of material science, engineering and technology. Ullakko´s first paper in this field has been cited over 2600 times. Ullakko has founded two high technology companies (in 1997 and 2015). Ullakko is a member of several international professional organizations and he was a Member of the Council of Natural Sciences and Engineering of the Academy of Finland during 2016-2018.
Abstract:
Will update soon.
Title: Will update soon
Biography:
Bruce McCord is an Analytical and Forensic Chemistry Professor at Florida International University. Dr. McCord received a BS in Chemistry with honors from the College of William and Mary in 1981, and a Ph. D. in Analytical Chemistry from the University of Wisconsin-Madison in 1986. His research interests involve forensic genetics, toxicology, and explosives residue detection. He has published over 100 peer reviewed papers and book chapters, and his research has been supported by the National Institute of Justice, the National Science Foundation, TSWG, the Department of Homeland Security and various industrial concerns.
Abstract:
Will update soon.
Title: Microplastic detection using impedance measurements in a microfluidic channel
Biography:
Vienna Mott completed her graduate degree in December of 2019 on the topic of impedance spectroscopy for the detection and identification of microplastics. With degrees in biomedical and mechanical engineering, Vienna specializes in microfluidic device design and fabrication. The concept that informed this research was named one of Time Magazines’ Top 100 Inventions of 2019.
Abstract:
Microplastics, polymer particles ranging in size from 1 um to 1 mm in diameter, are known to abundantly litter the oceans, but quantities and pollution trends have yet to be understood. In order to effectively quantify these data, an in-situ counting mechanism must be realized. This research studies microplastic discrimination using impedance spectroscopy. A microfluidic chip was developed using a low-cost fabrication method that used a simple soft lithography process. The chip allowed for the continuous injection of microplastic particles and the characterization of their induced impedance change. An increase in capacitance was observed when the microchannel was filled with water versus silicone oil versus air, validating finite element electrostatic simulations. A 7.5 fF increase in capacitance between water and air was observed, and silicone fell between these values, both theoretically and experimentally. From simulations, we determined that one microplastic particle (48 um radius in a 50 um radius channel) would result in 0.06 fF of capacitance change in air and 0.15 fF of capacitance change in water. This is not measureable with the current geometry and instrumentation. Recommendations are made for changes to device design to increase sensitivity, and estimates are made for the required resolution to achieve single particle detection. Notably, this was the first study to explore microfluidic impedance detection of microplastic particles in the ocean.
Keynote Session:
Title: Artificial intelligent point-of-care tools for rapid diagnostics of trauma
Biography:
Deniz Vurmaz is a doctoral candidate in the Department of Chemical and Biomolecular Engineering Department at NYU-Tandon, studying under Prof. John T. McDevitt. Her research is developing and integrating innovative diagnostic approaches to advance human health, focusing on programmable bio-nano-chip systems for multi-organ failure. Beyond basic science, she is a veteran of entrepreneurial competitions, having already won a NYU Green Grant and an award from the AABE for her team idea. Her goal is to be a bridge between academia and industry and, therefore, she has been preparing herself in this capacity. Before even joining NYU, Deniz was a project manager at an international renewable energy company. Using her leadership and entrepreneurship skills, she and her team established a start-up called “Lost-Bytes,†a data-driven food-waste management and renewable energy company that designs and employs Artificial Intelligence solutions to old school machines. She lectures in high profile renewable energy gatherings, such as the recently held Exxon-Mobil’s Energy Day. As the team leader, she has also been selected to NSF’s I-Corps Program and recently has funded by this program. She takes Launchpad Business classes upon becoming a member of GreenFeen Cooperative, which collects food-waste and composts in Bronx. Deniz grew up in Turkey and now lives in NYC.
Abstract:
Today’s healthcare delivery system focuses on late-stage disease diagnosis and, as a consequence, results in exceptionally high costs with poor outcomes in far too many cases. Recent developments in the -omics disciplines are starting to provide promising signatures of early disease detection. Likewise, advances in microfluidics, nanoscience, engineering, and artificial intelligence have the potential to drastically improve diagnostic systems. The need for rapid identification of organ failure after an accident is vital for immediate diagnosis, followed by the most relevant medical treatments.
In the quest for fast identification of organ failure, the key is rapid and accurate detection of pertinent biomarkers that are facilitated by the diagnosis of organ injury, the severity of trauma, and the potential for complications of hemorrhage. A comprehensive specialized treatment of the victim at a trauma care service is crucial within an hour of the incident for enhanced survival. At the same time, the rapid diagnostics followed by the appropriate therapies are a significant driver of healthcare costs. In fact, in the United States, approximately 35 million people are treated every year for trauma injuries which translates into one hospitalization every 15 minutes. At an annual cost of $67.3B, trauma is the 3rd most costly medical condition, behind heart disease ($90.9B) and cancer ($71.4B). Despite these facts, a highly effective point-of-care diagnostic device with analysis capabilities that facilitate the treatments is still profoundly absent. Our goal is to address this need by designing and implementing a highly affective chip-based detection system by integrating a wide variety of biomarkers. Using the selected biomarkers: CRP, Myoglobin, Complement 5, HMGB-1, Cystatin-C, N-GAL, L-FABP, Protein C, Properdin, D-Dimer, we developed a novel application of a universal chip-based sensor platform thereby enabling real-time, multiplexed, quantitative screening of trauma related biomarker panels with polymer based micron size sponge biosensors. Furthermore, the quantitative results generated is utilized to train machine learning algorithms to facilitate an intuitive and versatile Trauma ScoreCard that could effectively be used by the healthcare practitioners. The diagnostic tool includes a sensor module involving a single use, credit card-sized plastic cartridge employing a sample input port, microfluidics module, reagent blisters, biomarker array, waste reservoir, and high specificity antibody reagents.
Title: Direct fabrication of fluctuated walls in ceramic tubes by lithographic additive manufacturing
Biography:
Soshu Kirihara is a doctor of engineering and a professor of Joining and Welding Research Institute (JWRI), Osaka University, Japan. In his main investigation “Materials Tectonics†for environmental improvements of “Geotechnologyâ€, multi-dimensional structures were successfully fabricated to modulate energy and materials flows effectively. Ceramic and metal components were fabricated directly by smart additive manufacturing, design and evaluation (Smart MADE) using high power ultraviolet laser lithography. Original stereolithography systems were developed, and new start-up company “SK-Fine†was established through academic-industrial collaboration.
Abstract:
Ultraviolet laser lithography was newly developed as a direct forming process of fine ceramic components with micro geometric patterns. As additive manufacturing techniques, two dimensional cross sections were created through dewaxing and sintering by UV laser drawing on spread resin paste including ceramic nanoparticles, and three dimensional composite models were sterically printed by layer laminations and interlayer joining. Alumina particles of 300 nm in average diameter were dispersed in to photo sensitive liquid resins at 50 % in volume fraction. The resin paste was spread on a glass substrate at 50 μm in layer thickness by a mechanically moved knife edge. An ultraviolet laser beam of 355 nm in wavelength was adjusted at 10 μm in diameter and scanned on the surface. Irradiation power was increased to 1.0 W for enough solidification depth. The half wavelength of the incident ultraviolet ray should be comparable with the nanoparticles gaps in the resin paste, and electromagnetic field can be resonated and concentrated through Anderson localization. In this investigation, through computer aided smart manufacturing, design and evaluation (Smart MADE), fluctuated patterns were introduced into inner walls of micro tubes to modulate liquid and gaseous flows effectively.
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Oral Session 1:
- Oral Session 1
Chair
Alexander N. Govyadinov
HP Inc, USA
Title: A flexible and highly conducting bioelectrode utilizing nanostructured ceria for detection of Vitamin-D in serum sample
Biography:
Deepika Chauhan is currently pursuing Ph.D from Special Centre for Nanoscience, Jawaharlal Nehru University New Delhi-India. Deepika Chauhan has completed her Master in chemistry from Panjab University Chandigarh in 2010. She is currently working on synthesis and modification of different nanomaterials for biomedical applications including biosensor, bioimaging and drug delivery. She has developed different biosensing platforms for detection of Vitamin-d3. In this regard she has published three research papers in reputed journals and filed one patent.
Abstract:
Vitamin-D (Vit-D) deficiency is a worldwide health problem and the conventional techniques available for monitoring its status are expensive, time consuming and required centralized labs. The biosensor are good alternative over these techniques and in the present study we have fabricated nanostructured ceria (nCeO2) based bioelectrode for detection of 25-Hydroxy Vitamin-D3 (25-OHVD3). For bioelectrode fabrication, carbon cloth (CC) taken as a substrate as it is highly conductive, flexible, economical, easily available and eco-friendly material that can replace other substrates such as conductive glass substrate (ITO & FTO) and glassy carbon electrode etc. nCeO2 were synthesized using hydrothermal route and characterized through X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopic, transmission electron microscopy (TEM), Raman spectroscopy and Zeta potential measurements. The average size of nCeO2 was found to be 18.19±2.96 nm and the surface charge obtained by zeta potential measurement was -2.4 mV. The anchoring of nCeO2 onto CC was done by electrophoretic deposition method and we get a uniform and tightly deposited nCeO2/CC electrode. nCeO2/CC electrode was modified with biomolecules (anti-25VD3 and BSA) to fabricated the bioelectrode (BSA/anti-25VD3/nCeO2/CC) for detection of 25-OHVD3. The fabricated bioelectrode were characterised through scanning electron microscopy (SEM) atomic force microscopy (AFM) techniques. Further, the response study of bioelectrode as a function of 25-OHVD3 concentration revealed a linear detection range of 1-160 ng mL-1 with sensitivity of 2.08 µA ng-1 mL cm2, detection limit of 4.63 ng mL-1 and response time of 15 minutes. The validation study conducted on BSA/anti-25VD3/nCeO2/CC bioelectrode was in good agreement with the results obtained from ELISA of serum samples collected from healthy persons.
