Tissue regeneration (TR) is currently one of the most challenging biotechnology unsolved problems. Tissue engineering (TE) is a multidisciplinary science that aims at solving the problems of TR. TE could solve pathologies and improve the quality of life of billions of people around the world suffering from tissue damages. New advances in stem cell (SC) research for the regeneration of tissue injuries have opened a new promising research field. However, research carried out nowadays with two-dimensional (2D) cell cultures do not provide the expected results, as 2D cultures do not mimic the 3D structure of a living tissue.

Some of the commonly used polymers for cartilage regeneration are Poly-lactic acid (PLA) and its derivate as Poly-L-lactic acid (PLLA), Poly(glycolic acids) (PGAs) and derivate as Poly(lactic-co-glycolic acids) (PLGAs) and Poly caprolactone (PCL). All these materials can be printed using fused deposition modelling (FDM), a process in which a heated nozzle melt a thermoplastic filament and deposit it in a surface, drawing the outline and the internal filling of every layer. All these procedures use melting temperatures that decrease viability and cell survival. Research groups around the world are focusing their efforts in finding low temperature printing thermoplastics or restricted geometries that avoid the contact of the thermoplastic and cells at a higher temperature than the physiologically viable. This has mainly 2 problems; new biomaterials need a long procedure of clearance before they can be used in clinical used, and restrictions in geometries will limit the clinical application of 3D printing in TE.

To prevent the Cancer and rise the quality of life for Cancer Patient. The Aids of Pharmaceutical development of Cancer. The Cancer know from 1600 B.C. But humans have no awareness about Cancer. But it became more vital when research began (18th& 19th Century). In 2002, 10 million people are affected by Cancer and by 2017 it increased to 15 million. It is mostly seen in people of age above 15 years, among them 7.4 million cases are of men and 8.7 cases are of women. The scientists expect that this range may increase to 24 million in the future.

Lung Cancer was most common Cancer with Worldwide contribution of 13% of the total population. Breast Cancer, which is mostly seen among women is the second most common Cancer with 1.7million cases. The drugs that are developed to treat different types of cancers are Cytotoxic Drug, Targeted Drug, Bave in 2014 Bavenico, Kymriah, Kisqali, Nerlyx, Rydap, Bosponsa, Tafinlar, Mekinist Opdivo.12%cancers are caused due to virus

Chemotherapy is one of the most widely used therapy to treat cancer.The therapy include Targeted therapy &Immune therapy or Gene therapy that changes the “T” cells of the patient in the lab to make them more effective on cancer.From 18th&19th century the cancer patients increased widely throughout the world from 10-15 million, with this the pharmaceutical aids of life sciences also increased. Among all types of cancers only 4 types of cancers are dangerous. Some of the cancers are treatable were as some are under research .Herpes Virus is used to treat Brain Cancer

Progress toward the development of efficacious therapies for Alzheimer’s disease (AD) is halted by a lack of understanding early underlying pathological mechanisms. Systems biology encompasses several techniques including genomics, epigenomics, transcriptomics, proteomics, and metabolomics. Metabolomics is the newest omics platform that offers great potential for the diagnosis and prognosis of neurodegenerative diseases as an individual’s metabolome reflects alterations in genetic, transcript, and protein profiles and influences from the environment. Advancements in the field of metabolomics have demonstrated the complexity of dynamic changes associated with AD progression underscoring challenges with the development of efficacious therapeutic interventions. Defining systems-level alterations in AD could provide insights into disease mechanisms, reveal sex-specific changes, advance the development of biomarker panels, and aid in monitoring therapeutic efficacy, which should advance individualized medicine. Since metabolic pathways are largely conserved between species, metabolomics could improve the translation of preclinical research conducted in animal models of AD into humans. A summary of recent developments in the application of metabolomics to advance the AD field is provided below.

In the new generation, the internet has become an important tool for education, entertainment, communication and information-sharing. At the same time the rise of new generation gadgets, the risk of Internet Addiction (IA) is emerging as a significant behavioral addiction pandemic to be tackled worldwide. Excessive internet usage in university students results not only in psychological but physical problems which leads to deterioration in their academic performance. Thus it is imperative to find out the magnitude of IA and its association with sleep and depressive symptoms.

A cross-sectional study was conducted to select appropriate samples of 211 students using systematic random sampling method.  Bivariate and multiple regression analysis were performed to find out significant association of different possible factors with IA.

Prevalence of internet addiction was estimated as 23.2% in life experienced among MBBS and BSc. Nursing students. Among those who were addicted to the internet, 32.7% were suffering from some form of clinical insomnia and 42.9% were suffering from some form of major depression. Hence those who were addicted to the internet were 7 times more likely to suffer from insomnia (p<0.001) and 4 times more likely to suffer from major depression (p<0.001). However no such significance was found between internet addiction and the socio-demographic profile. Also, it was found that internet addicted students were 3 times more likely to have problematic alcohol use. The association between internet addiction and alcohol use was also statistically significant (p<0.05). In our study we found the prevalence of internet addiction to be quite high and a significant association was seen between internet addiction, insomnia, depression and problematic alcohol use.The proper counseling and timely rehabilitation can be provided to those who are in need.

In 1973 the American Spinal Injury Association made the International Standards for Neurological Classification of Spinal Cord Injury (ISNCSCI). In this clinical review our patient was classified after the vertebral fixation surgery with a ASIA-A scale injury after suffering a fracture and luxation at T-12-L1, having total spinal cord section (Fig.A). Based on the research made by Sergei Paylian, PhD on animal models [1] and the safety use of allogeneic MSCs demonstrated on multiple animal models applications [11,12], we decided to apply a experimental translational medical protocol based the research and the previous outcomes obtained by Hamid and MacEwan [8,9] and decided to customize it exclusively to our patient based on the clinical evidence and personalizing the therapy on evidence. The medical team designed an ambulatory method utilizing a C-arm to apply the allogeneic MSCs in situ and using a intrathecal (subdural) catheter using a slow pump release system for the rest of the biological material with an optimum tolerance and minor side effects (mild fever, miyalgias and headache) on the first 48hrs hour after application. The experimental use of mRNA Bioquantine® was well tolerated with its purified form (intra and extra-oocyte liquid phases of electroporated oocytes [6]) showing to be well tolerated by the patient without any anaphylactic reaction. The current clinical report is meant to demonstrate the beneficial changes with the use of Bioquantine® and its administration in a patient with a severe SCI offering a possible optional therapy and potential neuroregeneration in this clinical condition. Mesenchymal stem cells (MSCs) are ideal for cell-based therapy in various inflammatory diseases because of their immunosuppressive and tissue repair properties. Moreover, their immunosuppressive properties and low immunogenicity contribute

to a reduced or weakened immune response elicited by the implantation of allogeneic MSCs compared with other cell types, allogeneic MSCs are a promising option because of their low immunogenicity and immunosuppressive and tissue repair capabilities. The positive findings throughout the evolution of our protocol for spinal cord injury with the obtained results at this stage is a promising scientific based and evidence based medicine protocol that can be offered in the near future as an option for severe SCI patients. The functionality of the RestoreSensor® SureScan® by providing the electric stimulation fortifies the medical outcome and has given the patient the confidence to perform his physical rehabilitation with more energy for a longer time by the increase or decrease of the intensity of it according to the type of exercise regulated by the control-battery he handles. At this date, after 8 intrathecal applications of allogeneic MSCs and Bioquantine® in situ combined together we have got the following outcomes: an improvement in sensitivity, strength in striated muscle and smooth muscle connection by increased muscle mass (Fig.B) and sphincter

control, at 23 months after the first regenerative therapy and 12 months after the placement of RestoreSensor® the patient is showing an evident improvement on his therapy of physical rehabilitation (legs movement and control of them) having the following movements reported by the physical therapist: a)hip: adduction and external rotation, extension, abduction, internal rotation; b)knee: flexion; c)toe: MP and IP extension, also reporting an easier and functional crawling forward and backwards, and since 3 months ago the patient is capable to stand on his knees for 2 or more minutes without any support (Fig. C) and taking small steps on his knees forward and backwards for the first time in his process, showing a progressively important functionality on both limbs, voluntary movement at both feet and an increase in sensory perception.

There is increasing evidences that favor the prenatal beginning of schizophrenia. These evidences point toward intra-uterine environmental factors that act specifically during the second pregnancy trimester producing a direct damage of the brain of the fetus [1]. The current available technology doesn't allow observing what is happening at cellular level since the human brain is not exposed  to a direct analysis in that stage of the life in subjects at high risk of developing schizophrenia. Methods. In 1977 we began a direct electron microscopic research of the brain of fetuses at high risk from schizophrenic mothers in order to finding differences at cellular level in relation to controls. Results. In these studies we have observed within the nuclei of neurons the presence of complete and incomplete viral particles that reacted in positive form with antibodies to herpes simplex hominis type I [HSV1] virus, and mitochondria alterations [2]. Conclusion. The importance of these findings can have practical applications in the prevention of the illness keeping in mind its direct relation to the aetiology and physiopathology of schizophrenia. A study of the gametes or the amniotic fluid cells in women at risk of having a schizophrenic offspring is considered. Of being observed the same alterations that those observed previously in the cells of the brain of the studied foetuses, it would intend to these women in risk of having a schizophrenia descendant, previous information of the results, the voluntary medical interruption of the pregnancy or an early anti HSV1 viral treatment as preventive measure of the later development of the illness.