Title: Expression levels of obesity - related genes in high fat diet (HFD) induced experimental animals: Impact of combina􀆟 on therapy of an􀆟 -obesity drugs

Biography:

P Venkataraman has completed his MPhil degree from department of Endocrinology, University of Madras, Chennai, India and awarded PhD degree at the age of 32 years
from the same department. At present he is working as an Assistant Professor in Department of Medical Research, SRM Medical College Hospital and Research Centre, SRM
University, Chennai, INDIA. He has an extraordinary ability in the fi eld of Molecular,

Abstract

Obesity is a global health problem and also a strong
risk factor for type 2 diabetes mellitus, cardiovascular
diseases and obesity genes play a major role in the central
regula􀆟 on of energy balance. Orlistat (Xenical, Alli) is a
synthe􀆟 c an􀆟 -obesity drug approved by European Medicines
Associa􀆟 on (EMA) and Food and drug administra􀆟 on
(FDA). But studies shown, orlistat supplementa􀆟 on causes
severe liver and kidney injury. Melatonin, an indolamine,
produced by the pineal gland plays a major role in circadian
rhythm and one of the powerful an􀆟 oxidant. Literatures
shown, it also exerts control over metabolic func􀆟 ons that
determine fat accumula􀆟 on and obesity and it also protects
environmental toxicants induced liver and kidney injury.
Garlic (Allium sa􀆟 vum L.), has been used as medicinal and
alimentary medicine from ancient period and these has
been used extensively worldwide especially in countries
like India. Studies reported garlic and its cons􀆟 tuents have
shown the various biological ac􀆟 vi􀆟 es especially against
cardiovascular disease due to its blood lipid-lowering, an􀆟 -
thrombosis, an􀆟 oxidant eff ects and infl ammatory response.
Diallyl trisulphide (DATS), a natural product from garlic
have gained a􀆩 en􀆟 on in many biological ac􀆟 vi􀆟 es. Based
on literatures and to compensate the orlistat induced side
eff ects, we propose to study the expression levels of obesityrelated
genes in high fat diet (HFD) induced experimental
animals and also to see the impact of melatonin alone
and in combina􀆟 on with DATS supplementa􀆟 on compared
to orlistat alone therapy. We studied mRNA and protein
expression of KSR2, IRX3, FTO and Neuropep􀆟 de Y by real
􀆟 me PCR and western blot method in liver, adipose 􀆟 ssue
and brain of experimental animals along with an􀆟 oxidant
and liver func􀆟 on parameters. Histological observa􀆟 ons
of liver and adipose 􀆟 ssue were also studied. Based on
results we found that both the melatonin and DATS alone
and its combina􀆟 on with orlistat therapy is effi cacy and
safety when compared to orlistat alone therapy. This study
determined the clinical usefulness of melatonin and DATS
as supplemental therapeu􀆟 c agents in obesity induced liver
and kidney dysfunc􀆟 on as well as in orlistat therapy and it
may help in providing the therapeu􀆟 c op􀆟 ons to tackle the
obesity without causing side eff ects.