Title: Development of an electrochemical immunosensor based on a competition assay for C-peptide detection in human urine

Biography:

Sharmaine Reintar, 25 years of age, was born in the Philippines. She finished her Bachelor studies by 2016 at the University of Northern Philippines and is a Registered Medical Technologist by profession. She has 1 year (2017-2018) of experience working in a clinical laboratory setting to analyze several body fluids in the body. She got her Masters degree in nanoscience and nanotechnology at the Universitat Rovira I Virgili, Spain by 2019.  She started her PhD study in 2020 at the Medical University of Graz, Austria, which is currently ongoing.

Abstract

Diabetes mellitus cases are reported to increase each year, affecting approximately 463 million of people worldwide by 2019 according to International Diabetes Federation [1]. C-peptide is a by-product of pancreatic beta cells, which is secreted in equal amounts along with insulin. One of the advantages of C-peptide is a longer half-life (30 minutes) compared to insulin (5 minutes) in the plasma. The presence of C-peptide in urine is closely linked to the metabolic situation, particularly reflecting insulin secretion [2]. In this multidisciplinary study, we aim to develop an electrochemical immunosensor based on competition approaches for the detection of C-peptide levels in urine samples. The method is based on antigen-antibody interactions that are specific for C-peptide.