Title: Electrochemically confined nanopore sensing: from single molecule to single cell

Biography:

Si-Min Lu is a Ph. D. student at East China University of Science and Technology under the supervision of Prof. Yi-Tao Long. She is currently focusing on single entities analysis based on the confined nanopore platform and wearable sensors.

Abstract

Nanopore represents a unique and versatile sensor to analyze and manipulate single entities. We are aiming to develop a single-entity analysis platform based on the elegant electrochemically confined nanopores. The key ideal is confining the enhanced electric field within the narrow aperture of nanopore to analyze functional and dynamic information of single molecule, single nanoparticle, and single cell. We also built a single molecule sensing interface within the glass nanopore by incorporating the metal film into the inner confined space of glass nanopore to achieve single molecule reaction and single biomolecule sensing. A wireless nanopore electrode based on the metal film coated nanopore was also developed, which poses a possibility to track the electron transfer process in a single cell, and monitor the electrocatalytic process. Furthermore, to address the limitation of conventional nanopore technology, an electrical-optical binding platform was also developed to couple ionic current signal with nano-optical signal. As a result, we successfully achieved aggregation-induced emission (AIE) manipulation in a single cell and single cell manipulation.