Dhaval Patel has his expertise in plant fungi interaction in improving the health of the agricultural soil. He deals with the biocontrol and phyto-enhancer activities of fungi and also has expertise in studies relating to phytohormones production, phosphate solubilization, ammonia production, siderophore formation, and also deals with cellulase and chitinase enzyme for biocontrol activity.
Abstract
Talaromyces pinophilus (MG011365) fungi belongs to family Trichocomaceae. The aim of current research was to explore different fungi as Plant Growth Promoting Fungi (PGPF). The study involves the isolation of different fungi from the rhizosphere of various agriculture farms. After isolation of T. pinophilus its’ ability to produce phytohormone was carried out (Indole Acetic Acid, IAA). IAA estimation was carried out using Salkowski reagent. T. pinophilus was allowed to grow in cultivation media (Potato Dextrose Broth, PDB) in which one was supplemented with tryptophan (TRP) and one without TRP (TRP a precursor for IAA production). Phosphate solubilization was carried out by growing T. pinophilus in pikovskaya’s media and latter estimated using stannous chloride method, showing decent solubilization of phosphate. Further enzyme assay for cellulase and chitinase were carried out. For cellulase enzyme production 1% carboxymethylcellulose (CMC) and for chitin enzyme production 1% colloidal chitin was used along with 1X MM9 agar media. Statistical analysis was carried out with Trichoderma longibrachiatum (MG011366) which is one of the most explored PGPF strain. Talaromyces pinophilus showed predominant effect as compared to Trichoderma longibrachiatum