The process of Identification and age estimation has its significant role in forensics. Tooth dentin contains l-form amino acids which is biologically active. In the process of age estimation this l-form amino acid is transformed into d-form amino acids. Tooth samples were taken from three distinct

age groups of personals. The percentage of age dependant non-enzymatic changes of L-form amino acids to D-form amino acids mainly aspartic acid is considered .Separation of enatiomers is performed by high performance liquid chromatography and composition is specifically identified by polarimeter. Sometimes in extreme conditions like burnt and

severely decomposed cases it is unable to elicit the identification. During this conditions tooth has its role in identification. The identification characteristic is due to its DNA content and age estimation by amino acid dating. A case of an army personal posted in higher ranges on international border during his duty hours though not posted at the scene of incident. The body was burnt to the extent that it could not be recognized .It is the DNA analysis which help to identify the officer; tooth being the best source of DNA than bone. In this case DNA from bone didn’t amplify suggesting that tooth sample is preferred than any other forensic evidence.