Title: Forensic DNA fingerprinting and sex mismatch : Chimerism based on Y-STR DNA determination analysis

Biography:

Abstract

DNA analysis   identifies an individual as well as   different species also . Forensic DNA   is a branch of forensic science that focuses on the use of genetic material   to answer questions pertaining to legal situations, including both criminal and civil. It is a   technique  comparing criminal suspects' profiles to DNA evidence so as to assess the likelihood of their involvement in the crime. Thus DNA Fingerprinting analysis aids in the personal identification for forensic purposes   and has always been a driving motivation for law enforcement agencies in almost all countries since its inception. The introduction of DNA markers (Y-STR) has allowed for greater precision and higher discriminatory power in forensic testing. A criminal/ person committing crime after Bone Marrow Transplantation are a rare situation but not impossible one. Keeping such situation in mind, a study was carried out to find out the best biological sample  to be used for personal identification especially in forensic situation. We choose female patient (recipient ) and a male donor. The pre transplant  sample (blood)  and post transplant samples (blood, buccal swab, hair roots) were collected from the recipient (patient). The same were compared with the blood sample of the donor using DNA FP technique. Post transplant samples were collected at different interval of time (15, 30, 60 and 90 days). The study was carried out using Y-STR kit at 23 loci. The results determined discusses the phenomenon of chimerism  and its impact on Y-STR . Hair sample was found the moist suitable sample which had no donor DNA profiling upto 90 days.