International Webinar on
Stem Cell Research
October 11, 2021
Scientific Program
Keynote Session:
Title: DJ-1 Can Replace FGF-2 for Long-Term Culture of Human Pluripotent Stem Cells in Defined Media and Feeder-Free Condition
Biography:
Dr. Julee Kim received her diplom degree in Biotechnology at the Westfaelisch Wilhelms University of Muenster in 2007. She graduated from the Westfaelisch Wilhelms University of Muenster with a PhD degree in Biology at the Max Planck Institute for Molecular Biomedicine in 2013. She completed her post-doctoral training at the University of California San Francisco and at Columbia University, Irving Cancer Research Center. She worked as a research assistant professor at the University of Ulsan College of Medicine, Asan Medical Center and as a research professor at CHA University. She has 8 publications in international journals. She presented papers in more than 10 national and international conferences.
Abstract:
Conventional human pluripotent stem cell (hPSC) cultures require high concentrations of expensive human fibroblast growth factor 2 (hFGF-2) for hPSC self-renewal and pluripotency in defined media for long-term culture. The hPSC culture media need to be changed every day partly due to the hFGF-2 thermal instability in solution at 37°C. It has been known that the binding site of human DJ-1 (hDJ-1), also known as PARK-7 is FGF receptor-1. In the present study, for the first time, we have demonstrated that recombinant protein human FGF-2 can replace hDJ-1 in the essential eight media to maintain the pluripotency of H9 human embryonic stem cells (hESCs) under feeder-free condition. After more than ten passages, H9 hESCS cultured with human FGF-2 or human DJ-1 successfully sustained the distinctive hESC morphology. Furthermore, H9 hESCs revealed high expression levels of pluripotency markers including SSEA4, Tra1-60, Oct4, Nanog, and Alkaline phosphatase. DNA microarray revealed that more than 97% of the 21,448 tested genes, including the pluripotency markers, Sox2, Nanog, Klf4, Lin28A, Lin28B, and Myc, have similar mRNA levels between the two groups. Karyotyping revealed no chromosome abnormalities in both groups. They also differentiated sufficiently into three germ layers by forming in vitro embryoid bodies and in vivo teratomas. There were moderate difference in H9 hESCS in both groups was shown in the real-time PCR assay using several pluripotency markers and three germ layer markers. The proliferation rate measured at different concentration of growth factors and the structural analysis of mitochondria using transmission electron microscopy demonstrated the distinguishable feature of H9 hESCs in two groups, namely hFGF2 and hDJ-1. On the whole, in-house made recombinant protein hDJ-1 can maintain the self-renewal and the pluripotency of H9 hESCs in a feeder-free system for long-term without alteration of their characteristics
Title: Malignant Tumor Regression Balancing Internal Energy and Chakras’ Energy Replenishment
Biography:
Huang Wei Ling, born in Taiwan, raised and graduated in medicine in Brazil, specialist in infectious and parasitic diseases, a General Practitioner and Parenteral and Enteral Medical Nutrition Therapist. Once in charge of the Hospital Infection Control Service of the City of Franca’s General Hospital, she was responsible for the control of all prescribed antimicrobial medication and received an award for the best paper presented at the Brazilian Hospital Infection Control Congress in 1998. Since 1997, she works with the approach and treatment of all chronic diseases in a holistic way, with treatment guided through the teachings of Traditional Chinese Medicine and Hippocrates. Researcher in the University of São Paulo, in the Ophthalmology department from 2012 to 2013. Author of the theory Constitutional Homeopathy of the Five Elements Based on Traditional Chinese Medicine. Author of more than 100 publications about treatment of variety of diseases rebalancing the internal energy using Hippocrates thoughts.
Abstract:
Spontaneous tumor regression was defined differently for many types of cancer by several researchers during the last century. Spontaneous regression is partial or complete disappearance of primary tumor tissue or its metastases in patients who have never been treated. According to traditional Chinese medicine (TCM), malignant tumor has a cause energies deficiencies and Heat retention. Purpose: to demonstrate that malignant tumor can regress in to benign or disappear completely only balancing internal energies, taking out Heat retention and replenishment the chakras energy deficiencies with highly diluted medications. Methods: Three clinical cases reports. All three patients with cancer diagnoses (case one: thyroid; case two: uterus; case three: lungs). All three patients were found to be at their lowest level of energy (through radiesthesia), rating one out of eight. Treatment consisted in reestablish the equilibrium between Yin, Yang, Qi, Blood and taking out Heat retention through Chinese dietary counselling, auricular acupuncture with apex-ear bloodletting, homeopathy according to the Constitutional Homeopathy of Five Elements Based on Traditional Chinese Medicine and crystal-based medication. Results: The first two case reports were cured of their cancer condition without any treatment by Western medicine, only with the treatment done. The third patient, though, was already under radiotherapy and chemotherapy but through the treatment previously described, the metastasis disappeared and he achieved a better physical and emotional health state. Conclusion: balancing internal energies and taking out Heat retention through Chinese dietary counseling, auricular acupuncture with apex ear bloodletting and replenishment the chakras energy meridians with highly diluted medications can induce malignant tumor regression according to these three cases reports. More studies should be done with more patients and with different kinds of tumors to have more data and confirmation of these results.
