Title: Identification of Bacterial Samples Using Laser Spectroscopy

Biography:

Walid Tawfik is a specialist in microbiology, currently he is working in the area of oncology. He completed his PhD in Biotechnology from Cairo University, Egypt. His research areas are mainly in Microbiology, oncology, cell culture, tissue culture.

Abstract

The laser-induced plasma spectroscopy (LIPS) technique was used to analyze two different species of bacteria, Escherichia coli (B1) and Micrococcus luteus (B2), placed on glass slides. Spectrolaser software was used to examine LIPS spectra. The glass substrate's LIPS spectrum was compared to bacterium spectra. In airborne bacterial samples, atomic emission lines were observed. Two carbon lines at 193.02 nm and 247.88 nm, as well as one hydrogen line at 656.28 nm, occurred in bacterial samples B1 and B2, with intensity different ratios. Bio-samples such as bacteria and cancer cells contain significant amounts of carbon and hydrogen. The results of a study of the LIPS spectra of samples in He and Ar atmospheres are also reported. In an Ar environment, Ni lines showed solely in the B2 sample. We may conclude from the current experimental findings that the LIPS technique has a possibility in the detection and identification of different types of bacteria. Keywords: Laser-induced plasma, Escherichia coli, Micrococcus luteus Bacteria, elemental lines.