Title: Immunohistochemical distribution of androgen receptor in male and female Dwarf Hamsters’ (Phodopus roborovskii) genital tissues

Biography:

Zeliha Selamoglu is a Professor in Medical Biology department of Nigde Ömer Halisdemir University, Turkey. She earned her PhD in Biology from Inonu University, She has published over 90 peerreviewed journal articles with over 865 citations and many technical reports. She is a member of Society for Experimental Biology and Medicine: Associate Membership and European association for cancer research. She has served as Editorial Board member for many Journals. Her research Interest focuses on Medical Biology, Molecular Biology, Biochemistry, Biotechnology, Oxidative stress, Antioxidants, Cancer.

Abstract

Androgens are essential for the normal development and functional maintenance of reproductive organs. Testosterone is the principal circulating androgen; it is synthesized mostly as androgenic precursors in the gonads. Androgens exerts their effects on target tissues mainly through the androgen receptor (AR). In this study, AR localization was investigated in female and male dwarf hamsters’ genital tissues. In the present study, 6 female and 6 male animals were used. After anesthesia, the tissues to be worked were quickly removed from the body and fixed in 10% buffered neutral formalin, and embedded in paraffin. Sections were stained after microwave antigen retrieval for immunohistochemistry. Although AR positive immunostaining was observed only in cell nuclei in testis, caput epididymis, prostate gland and vesicle seminalis tissues, it was observed in cytoplasm or nucleus of the cells in the ovarium and oviduct tissues. In testis AR immunostaining was detected in the nuclei of peritubular myoid cells, pericytes, Sertoli cells, and Leydig cells, but not in germ cells.  Positive immunohistochemical staining for the AR was observed in prostate and seminal vesicle cells such as luminal epithelial cells and stromal cells. Although, in ovarium some of the granulosa and theca cells were showed nuclear staining others were showed cytoplasmic staining. AR localization in dwarf hamster male and female genital tissues was similar to other species. The presence of AR in genital tissues supports the idea that androgens are essential for these tissues.