Title: Study of Immunomodulatory activity of Terminalia arjuna (roxb) bark on in-vitro neutrophils

Biography:

Kamal Singh Rathore has completed his PhD at the age of 37 years from MLS University, Udaipur. He is the Head of Department of Pharmaceutics, at BNIPS, Udaipur a premier pharmacy institute. He has published more than 20 books and 100 papers in reputed journals and has been serving as an editorial board member of repute journals.

Abstract

Immunomodulation is any procedure which can alter the immune system of an organism by interfering with its functions; if it results in an enhancement of immune reaction is named as immunostimulation and primarily implies stimulation of non-specific system i.e., stimulation of the function and efficiency of granulocytes, macrophages, complement, certain T-Lymphocytes and different effector substances.  Immunosuppression implies mainly to reduce resistance against infections, stress and may be because of environmental or chemotherapeutic factors. The objective is to explore the possibility of using traditional medicines as an immunomodulatory agent with proper scientific account. Chemotherapeutic agents available today have mainly immunosuppressive activity.  Most of them are cytotoxic and exerts a variety of side effects. This has given rise to stimulation in the search for investigating natural resources showing immunomodulatory activity. Terminalia arjuna has been documented to produce immune promoting effect. However, detailed phytochemical investigations of leaves for in-vitro immunomodulatory activity have not been scientifically documented. Here in this study the extracts of petroleum ether, ethyl acetate, chloroform alcohol and cholorofrm water of Terminalia arjuna (Roxb) bark was investigated for immunomodulatory activity by assessing Nitroblue tetrazoldium test, phagocytosis of killed Candida albicans, candidicidal assay, neutrophil locomotion and chemotactic assay.  All the extracts were given at dose of 5 mg/ml, 10 mg/ml, 25 mg/ml, 50 mg/ml and 100 mg/ml.  Results of in-vitro immunomodulatory activity lead to the conclusion that the chloroform extract and isolated compound of Terminalia arjuna bark showed predominantly significant activity on in-vitro human neutrophils in all parameters as compared to other extracts.