Title: Antimicrobial Efficacy of Polyherbal -made Toothpaste and Mouthwash against Oral Pathogens: A Randomized Clinical Trial

Biography:

1. Batool Sadeghi-Nejad Graduated of bachelor degree (B. Sc.) in the field of public health from Isfahan University of Medical Sciences on Feb. 15 1988
2. Holder of master degree (M. Sc.) in the field of Medical Parasitology from Isfahan University of Medical Sciences on April 30 1997.
3. Holder Ph.D. degree in Medical Mycology from Botany department of Pune University, India on Feb. 2008..  She is members of Scientist Academic Institution, Abadan School of Medical Sciences, Abadan, Iran (Assistant professor, Ph.D Medical Mycology). She has published more than 24 papers in reputed journals and has been serving as an reviewer board member of repute.          

Abstract

Background: The aim of this study was to evaluation in vivo antimicrobial effects of polyherbal-made toothpaste and mouthwash to form effervescent tablet containing the extracts of Artemisia dracunculus, Satureja khuzestanica and Myrtus communis against oral pathogenic microorganisms (Streptococcus sanguinis, Streptococcu salivarious, Streptococcu mutans and Candida albicans).

Methods: This study was clinical trial without control (Before – After test) that were performed on 20 volunteers, healthy with normal gums without bleeding or inflammation. Twenty volunteers will be given herbal toothpaste and asking from them to wash their mouths by sterile deionized water, pour the contents of their mouth into sterile container as samples of before test. Then asked them to use brushing their teeth with herbal toothpaste. The next morning again asked from same volunteers to wash their mouths by deionized water for one minute and pour the contents of their mouth into another sterile container as sample of after test. Then the samples transferred to laboratory immediately. Aliquots of 100µl of each sample inoculated on petri dishes containing blood agar for bacteria and Sabouraud dextrose agar for Candida albicans. Then colonies counted before and after test, separately. This process were repeated for the herbal effervescent tablets mouthwash by other 20 volunteers with same condition.

Results:

According to the laboratory results, the Candida albicans was isolated from all the voluntaries. The scores of colony growth decreased significantly between before - after test during the studies (p<0.001).                                                                                                      

Conclusion: The results were revealed that polyherbal toothpaste and mouthwash could be effective for prevention of prevention of dental caries in future.