Title: Public Health Implication of Brucella abortus Strain-19 Vaccine in Milking Cows: A Possible Shedding of the Brucella organisms in Milk

Biography:

Dr. A. S. Saidu has completed his PhD at the age of 32 years from LUVAS University, India and a Senior Lecturer Medicine. He is the focal person in University of Maiduguri for reporting zoonotic diseases to the Nigeria Centre for Disease Control, a National body saddled with the responsibilty of detecting and responding to emerging Disease outbreaks. He has published more than 30 papers in reputed journals and has been serving as an editorial board member of reputable journals. I have DVM, MPH degrees and other specialty skills such as IPC, AMR, Epidemiology, Zoonoses, etc., with good interpersonal skills.

Abstract

The presence of Brucella DNA in milk samples was detected by BCSP31 gene-targeted PCR and by amplification of IS711-genes by conventional PCR. Confirmation of the PCR positive samples was done by qPCR using TaqmanR assay. A total n=120 milk samples were collected from the vaccinated milking cows during days post-vaccination (DPV) at 30PV, 60PV, 90PV and 120PV (n=10 each). Some were found positive for both Brucella genus and species-specific genes in subcutaneously (s/c) vaccinated cows. Significantly higher positives were detected by the qPCR. BCSP31 sequence was deposited at GenBank NCBI and assigned accession no. MK881173-6. The presence of brucella organisms in milk of vaccinated cows revealed the risk of zoonotic transmission of brucellosis to humans, if raw milk was consumed and the public health threats associated with that. These results strongly suggested that use of both conventional PCR and qPCR techniques could lead to more reliable diagnosis of brucellosis from vaccinated bovine milk samples. There is a public health of contracting brucellosis in milking cows. Key words: Brucella abortus, Cows, Milk, Strain-19, Vaccine, Zoonosis.