Title: Immunization of goat with recombinant B2L and Kisspeptin-54 DNA vaccine inhibits fertility via disrupting the hypothalamic-pituitary- testicular axis

Biography:

Teketay Wassie is a PhD at Huazhong Agricultural University, China. He has served more than 4 years as a University teacher at Assosa University, Ethiopia. He has over 10 publications published in international journals.

Abstract

Statement of the Problem: Kisspeptin, encoded by the KISS1 gene with its cognate receptor GPR-54 is recognized as an upstream orchestrator in the hypothalamic-pituitary-gonadal (HPG) axis.  Despite kisspeptin role as a gatekeeper of gonadotropin-releasing hormone (GnRH) secretion is well known, the effect of kisspeptin immunization on HPG axis in goat is not yet studied. The purpose of this study was to investigate the effect of recombinant orf virus envelope protein (B2L) and kisspeptin-54 DNA vaccine on HPG axis. Methodology: A total of 15 male white Yichang goats were equally and randomly assigned in to one of the following three groups: PBK-asd immunized, PVAX-asd treated (control) and surgically castrated. Bucks assigned in the PBK-asd and control group were intramuscularly immunized with 1mg/dose of PBK-asd DNA vaccine and PVAX-asd plasmid, respectively. Finding: Administration of recombinant B2L and Kisspeptin-54 DNA vaccine is shown to produce significant antibody responses to kisspeptin and decreased serum levels of luteinizing hormone (LH) and testosterone levels as compared to control group (p<0.05). The current recombinant vaccine caused testicular atrophy in vaccine treated animals and prevented spermatogenesis. In contrast, the surgical castrated group showed significantly higher serum FSH and LH levels as compared to control group (p< 0.05). Furthermore, our data showed that PBK-asd immunization altered HPG axis via reducing hypothalamic androgen receptor (AR), G protein-coupled receptor (GPR54), gonadotropin-releasing hormone (GnRH); pituitary GnRH receptor, follicle stimulating hormone beta (FSHb), luteinizing hormone beta (LHb), and testicular FSH and LH receptors. In contrast, surgical castration significantly increased mRNA expression of all reproductive genes detected in HP axis except AR (p< 0.05). Conclusion & Significance: It is concluded that PBK-asd immunization inhibits fertility via perturbing the genes and receptors expression, and hormones production in the HPG axis. This research provide insight for the use of kisspeptin vaccine in the modulation of fertility.