Biography:

Mustafa A. N. AL-Fayyadh is a student studying Master of Science in Biotechnology at the department of Biotechnology, Eskisehir Technical University which was under name of Anatolia University.He has had a 7 months training in Biotechnology and Molecular biology at the University of Malaya in Malaysia at The Centre for Research in Biotechnology for Agriculture (CEBAR) under the supervision of Prof. Dr. Jennifer Ann  Harikrishna.

Abstract

Fusarium oxysporum f. sp. cubense (Foc) is a disease that attacks bananas causing great economic losses to banana production worldwide. Conventional banana breeding to introgress resistance from wild relatives to susceptible cultivars is complicated and takes a long time. Markers assisted selection is a modern tool thought to over some barriers of banana breeding by increasing the breeding efficiency and shortening the breeding period. The aim of this study was to genotype two unrelated mapping populations developed Foc race 1 resistance and identify markers for analysis of Foc race 1 resistance QTL. Two F₁ mapping populations were developed for Foc race 1 resistance: First population of 140 hybrids, by crossing a resistant Monyet with a susceptible Kokopo and the second of 138 hybrids by crossing a susceptible Mshale with a resistant Calcutta 4. The parents contrasting for Foc race 1 resistance were screened using IRAP, ISSR, and SSR markers. IRAP primer GyLTRev had the highest polymorphism of 76.9% between Mshale and Calcutta 4 parents and 69.2% among their F₁ hybrids and  64.3 % between Monyet and Kokopo parents of and 42.9% their F₁ hybrids among all the primers. One major QTL associated with Foc race 1 in an F₁ population of Monyet x Kokopo was mapped at linkage groups 1 (LG1) with a  LOD score of 4.0 using 32 polymorphic markers. Results in this study should be useful for further elucidating the mechanism of resistance to Fusarium wilt and in the development of molecular markers.