Title: Phylogenetic analysis of native Lactobacillus strain isolated from Vaccinium floribumdum kunth using 16S rDNA gene

Biography:

Celia Vargas is pharmaceutical chemist. She has completed her MSc in pharmaceutical biotechnology from University of Bologna, Italy. She is doing a PhD in biological science and engineering from National Agrarian University, Peru. Presently, she is the Professor at Faculty of Pharmacy and Biochemistry from University San Marcos, Peru and member at Centro Latinoamericano de Ensenanza e Investigacion en Bacteriologia Alimentaria, CLEIBA.

Abstract

The present study investigated berries that grown in a wild way in Peru above 3500 m.a.s.l. and these have become medicinal importance due to its high concentration of antioxidants, and several species of Lactobacillus, which are part of their native flora, could be a valuable tool in order to increase natural benefits in different foodstuff. This study aims to analyze phylogenetics and taxonomy based on 16S rDNA gene allows differentiation at species level. The bioinformatics analysis (BLAST and reconstruction of phylogenetic trees), found the genus Lactobacillus, Leuconostoc and Weissiella. Some strains showed similarity about 100% with Weissella cibaria KACC 11862, Leuconostoc mesenteroids subsp. dextranicum and Leuconostoc mesenteroids subsp. mesenteroids located in the same clade in the phylogenetic tree, which would confirm its phylogenetic approach. The native strains of the genus Lactobacillus showed similarity with L. plantarum, L. paracasei, L. fermentum and L. pentosus, while others native strains of same genus were located in different clades, confirming a probable diversity of species. Due to these results it is suggested to carry out the phylogenetic analysis with more specific genes for their taxonomic location.