World Congress on

Chromatography

Scientific Program

Keynote Session:

Meetings International -  Conference Keynote Speaker Magdalena Dabrowska photo

Magdalena Dabrowska

Warsaw University of Life Sciences, Poland

Title: Effect of microwave pretreatment method on the quality of straw pellets and the agglomeration process

Biography:

Magdalena Dabrowska is currently in the Department of Agricultural and Forest Machines. she had published nearly 6 publications including "INFLUENCE OF STEAM EXPLOSION ON MATERIAL PROPERTIES UNDER PRESSURE AGGLOMERATION".Her skills and expertises are in Renewable Energy technologies, Energy conversion and Biomass.

Abstract:

Considering the changes in lignocellulose structure, alternative heating is a competition for conventional heating. Radiation causes delignification and partial removal of hemicellulose and increased carbohydrate hydrolysis. Standard heating works on the surface heat exchange whereas microwaves cause that heat is generated as a result of the object's influence in the electromagnetic field. In contrast to conventional heating methods, the effect of microwave heating is almost instantaneous, there is no time to wait for a source of warming up or cooling down. The aim of the work was to acquire a new knowledge and explain the effect of the use of microwaves and the addition of glycerin to lignocellulosic material from straw of different moisture content. The best compaction parameters were selected: 0.1 g mass of single sample, 140°C head heating temperature, 60 mm die thickness. The density of obtained pellets, pressure of compaction, compressive strength analysis and the net and gross calorific value of the material with the addition were determined. Studies have shown that the addition of glycerin to biomass is advantageous when the material is not pre-treated in the microwave. The best density and durability had pellets made of material with a moisture content of 20%.

Keywords: Microwave, Pretreatment, Agglomeration, Pellet, Calorific value

Meetings International -  Conference Keynote Speaker Chien Hsun Huang photo

Chien Hsun Huang

Fudan University, China

Title: Uncovering history of angiosperm lineages using bioinformatics improves our understanding to the underlying mechanism in evolution

Biography:

Chien-Hsun Huang is now an associate researcher in Fudan University, China. He received his Ph.D. degree of Plant Biology in the Institute of Plant Biology, Department of Life Sciences, National Taiwan University, and started to study plant evolution in bioinformatics in his postdoc position. He accumulated his experiences in molecular biology, biochemistry and bioinformatics. He has involved in several government-granted projects of NSFC.

Abstract:

The advances of sequencing techniques greatly promote large amounts of datasets generated around the world, and make the bioinformatic approaches getting vital in the genomic era. Comprehensive comparative studies give us the opportunity to understand the basic mechanisms universal to most of the species but not just focused on some model species, and still allow us to identify the specified machinery in some particular organisms. A phylogeny is as a fundamental backbone required by almost all other researches to interpret their result correctly and make robust assumptions and hypotheses. Considering the importance of a robust phylogeny, we focus on resolving the relationships of angiosperm lineages, aiming to construct a framework for comparative studies in plants. We generated large numbers of transcriptomic datasets across plant families, and used nuclear genes as phylogenetic markers via a series of selection processes in order to eliminate the potential erroneous signals brought by some sequences. We used hundreds of low-copy or single-copy nuclear genes to reconstruct the phylogeny of Asteraceae, Rosaceae, Brassicaceae and other plant families. In our studies, we provide solid phylogenies of these families not only for the backbone but also some derived nodes, by using several approaches for a confirmative conclusion. The evolutionary history of morphologies can then be estimated (such as the evolution of fruit types), as well as the occurrences of gene/genome duplications. Cases of repeated/nested whole-genome duplications are found in some families, while many of them being located along the backbone nodes of that family. For morphological characters, parallel evolution was observed for some characters, while some others were not as flexible, which might indicate different roles or mechanisms for these characters.

Keywords: Angiosperms, Bioinformatics, Evolution, Genome, Phylogeny, Plant, Transcriptome

Oral Session 1:

  • Cancer Biology and Immunology | Biotechnology Applications
Meetings International -  Conference Keynote Speaker Jan Szopa photo

Jan Szopa

University of Wroclaw, Poland

Title: Optimizing flax raw products for tomorrows uses

Biography:

Szopa Jan, professor., biochemist/biotechnologist, MSc (1969), PhD (1972), DSc (1977), full Professor (1987). Head of Genetic Biochemistry Department at Wroclaw University. Awarded twice by Polish Biochemistry Society (1971, 1973), Polish Academy of Sciences (1991) and Polish Prime Minister (2009). Fellow of Humboldt Foundation (1978-1980, 1982-1983), Max-Planck Gesellschaft (1994-2004) and French Government (2001, 2002). Co-founder & Editor of Cellular and Molecular Biology Letters and member of Editorial Board of Plant Physiology and Biochemistry. Research interest: plant metabolism; transgenic potato and flax plants; chromatin arrangement ; plant fibers and natural compounds. Author and co-author of over 160 original publications.

Abstract:

Flax is a dual-purpose plant providing the fiber and oil. The principal use of fiber was for textiles manufacturing and oil for paints and varnishes. However, in the last decades devaluation of flax fiber in the world has been observed due to hardships associated with flax cultivation and processing, some disadvantages of flax fiber (poor elasticity, unpredictable quality) properties together with the appearance of cotton and synthetic fibers on the market. Recently, due to research findings the flax raw material appears to provide a variety of industrial and health benefits. For example manipulation of flavonoid genes (e.g. CHS, CHI, DFR, GT) expression significantly increases antioxidant potential and thus oil stability against oxidation and fatty acids composition.  Squalene accumulation (potent anticancer agent) was increased by silencing carotene synthesis. Unique flax fiber was obtained, by co- synthesis of polyhydroxybutyrate (PHB) with cellulose during fiber development. The PHB-fiber embedded in polylactide may serve as a scaffold for tissue engineering and has been shown to be useful as biodegradable implant. The unique application of flax product has been shown in chronic wound healing. Pre-clinical study revealed healing improvement of chronic ulcers upon treatment with wound dressing based on new fibers strengthened by supplementation with  activators derived from refined seed extract.

Meetings International -  Conference Keynote Speaker Ylfete Shatri Mucaj photo

Ylfete Shatri Mucaj

Kosova University Clinic Center in Prishtina, Kosova

Title: Acidum Ascorbicum and the other vitamins adjuvants in magistrial preparartions (DIADERM & ANTISEP-V) in the treatment of Acne in our professional practice

Biography:

Ylfete A. Shatri Mucaj, dermatovenerology and clinical  pharmacology specialist. For specialization of clinical pharmacology is one of the first generations for basic specialization in this field in Balkans. She finished post-graduation and specialization studies in the University of Novi Sad. Currently, she works in UCC in Prishtina from 1981; as a specialist of clinical pharmacology she has treated for 4 years patients with chemotherapy near Internal Clinic – Department of Hematology in UCC. She led for many years the laboratory for magisterial preparations in the Center Pharmacy and Dermatovenerology Clinic near CUU in Prishtina. She worked for some years in the Institute of Biochemistry near Diagnostic Center of CUU. Since 2001 she is working as a Clinical Dermatovenerologist and Pharmacologist in KDV and now leads Dermatological polyclinic and laboratory for magisterial preparations for several skin diseases and and aesthetic issues by which she treats patients in the polyclinic “GENTIANA GreLorGen”. She participated in many conferences and congresses in region and Europe with several scientific works in the field of clinical Dermatology, Oncology and Pharmacology and also as a lecturer of chemotherapy in the National Congress of Oncologists in Prishtina. She is a member of EADV and Kosovo Association of Dermatovenerologists and Oncologists.OMICS group contribute  (Sun Antonio 2014   and Chikago 2016;...est. )

Abstract:

The point of  presentation  in our  professional  experience is to Express th  importance  of  the acidum  ascorbicum (Vit.C) and  ther vitamins  adjuvanse  in our  magistrial  preparations  mentioned  belore  as  antioxydanse, epitelisanse,revitalisanse,revitlization and  mechanical  assistant  of  aktive  substances  action  in  magisterial  preparations  during  the  medical  treatment  of  acne  (even if  it  is an antibiotik,antiinflamator,keratolitic  or adstringents...).

The time  period of  treatment  with  mentioned  preparations  has  been  6   years, but  to see and  to observo  the  therapeutic  success we started  the  studying and  observacion  for  a  time  period  of   2  years  (January   2014 –January  2016).

The  number  of  the  treated   patients  was  1216,patients  of  different   ages  and  sex, and also  different  kind  of acne ,which  we  treated  with DIADERM  and ANTISEP-V. This  was  the firot  grup,and  also  we  had  a second  comparing  grup  of  350  patients of  different  ages  ,sex and different  kind  of  acne  but  who  were  treated  with  ther  magisterial  preparations  without Vitamin adjuvanses.

As  a conclusions, we  found  out  that  the firot  grup,treated  with  DIADERM  and ANTISEP-V had obviosly  more  success than the  second  group.

Key words: Adjuvans,vitamins,apply,acne,epiteliasing

Meetings International -  Conference Keynote Speaker Serena Diana Ghezzi photo

Serena Diana Ghezzi

Science Draw Graphic, Milan

Title: Graphic Communication of Scientific Research

Biography:

Serena Ghezzi obtained Veterinary Biotechnology degree, at the University of Milan, at 24 years old and Biology degree at the same University, at 30years old. She  spent10  years  in different renowned research institute;  having  published  24 papers, 1 book chapter and more than 40 posters presentations at  international  meetings she starts the Scientific  Graphic Consultant career. Founder of Science Draw Graphic in 2013, she is Scientific Graphic Consultant freelance and trainer of different graphic courses

Abstract:

Science Communication includes public communication of science-related topic to non experts but even communication among scientists (4). The last one isn't restricted to national or international meetings; all scientists communicate science everyday speaking with colleagues, bosses, students, customers but even reading a paper or writing a poster. Difficulties typify each situation and graphic language is a resource to elevate and enhance the message efficacy. Making visualizations is, already, integral to scientific thinking, indeed scientists imagine new relations, test ideas, and elaborate knowledge through visual representations (1). Graphic communication increase attention, understanding, memorization and ability to make decision (3); it engage people into physical and emotional challengers (2). This is why, also for scientists is very useful to know graphic language; especially for biotechnologists who often have to explain non visible mechanisms or methods. Scientists already rely on diagrams, graphs, tables, videos, photographs, and other images to make discoveries, explain findings, and excite public interest (1). Ainsworth et al, in 2011on the journal Science, discussed drawing efficacy to enhance engagement, to learn to represent in science, to reason in science, to learn strategy and to communicate (1).  More recently, Infographics (3) and Creative Research Methods (CRM) (2) are extensively investigated.  CRM consist of visual methods feature prominently in the group of items typified by workshops (e.g., renderings, maps, and collage making), although the variety of activities increases. Games, site visits, user-testing 3D models, mockups, prototype and scenarios are some of the types of participatory methods employed (2). I would like to speak about this underestimate and little know topic: discuss about its relevance; show an overview of graphic tools, their usefulness and applications field.  

Meetings International -  Conference Keynote Speaker Hossain Uddin Shekhar photo

Hossain Uddin Shekhar

Dhaka University, Bangladesh

Title: Understansing noncommunicable diseases

Biography:

Hossain Uddin Shekhar, is a promising professor presently working in the Department of Biochemistry and Molecular Biology, Dhaka University, Bangladesh. He did his PhD and post doctoral work in Tokyo Metropolitan University and the United Nation University, Tokyo, Japan, respectively. He worked as a visiting professor in Chubu University, Nagoya Japan in 2009. He is member of the editorial board of many national and internationally reputed journals at home and abroad. Dr. Shekhar works in the field of food functionality food safety, clinical Biochemistry and signal transduction.

Abstract:

Chitosan (a linear polysaccharide composed of randomly distributed β-(1-4)-linked D-glucosamine and N-acetyl-D-glucosamine) was extracted from dried waste prawn shell chitin (yield 15-17%) and later degraded by differential γ-radiation dosages (20kGy, 30kGy, 40kGy, 50kGy) of Co60 to see their effectiveness as a biodegradable preservative of perishable foods. Labeo rohita (Hamilton) or rohu fish and betel leaf have been chosen as representatives of perishable food samples (due to microbial infection). 10g/L, 50kGy irradiated chitosan acted as best antimicrobial agent for the fish sample after 6 days long observation. The sensory assessment showed the same chitosan sample kept the fish samples acceptable up to 16 days at (4±1) 0C compared to control sample. Total volatile base nitrogen (TVB-N) indicator shows control fish samples as acceptable up to 6th day (value of TVB-N <35), whereas 10g/L , 50kGy irradiated  chitosan treated fish samples were acceptable up to 14 days (28.79±4.2 mg/100g fish). pH of the fish flesh treated by the test chitosan sample showed value near to physiological level (7.18±0.4) up to 16 days. On the other hand, a 5 days observation period divulged , 5g/L, 40KGy irradiated chitosan exposed betel leaf having better appearance and least spoiled. This chitosan sample elicited maximum zone of inhibition in Salmonella (14.1±0.56mm), Shigella (15.26±0.33mm), Pseudomonas (14.85±0.21mm), Escherichia (12.89±0.26mm) and Xanthomonas (16.15±0.28mm). The same concentration of chitosan solution was able to completely inhibit the growth of A. niger, A. flavus, Rhizopus and Colletotrichum fungi in betel leaf.

Meetings International -  Conference Keynote Speaker Metin Turan photo

Metin Turan

Yeditepe University, Turkey

Title: Biotechnological M anagement to Prevent Wastage of P lant P roducts in S torage - Transportation Chain for the Consumer

Biography:

Metin Turan has completed his PhD from Ataturk University Soil Science Department. He is currently working as full Professor at Yeditepe University Genetics and Bioengineering Department. He has published more than 100 papers in reputed journals and has been serving as an Editorial Board Member of repute.

Abstract:

Today, due to the significant losses in post-harvest, preservation and transportation of fruit and vegetables, it is needed healthy alternatives for the effective and sustainable marketing of agricultural products. It is important to look at every stage of agricultural problems from production to the table. Various packaging materials can be used to protect food, and coatings are being developed as an alternative material.
 
In this study; biocompatible protective film and bio composite protective coatings that retard biodegradation and retard ripening, reduce water loss and respiration, prevent toxic effects that reduce microbial invasion and loss of taste on foods, retard nutrient depletion and also support plant vitamins and minerals.
 
For this purpose, a new generation synthesis product was used which contains various amino groups and benefits from the bacteriostatic -bactericidal action. 0, 150 ml,500 ml, 1000 ml, 1500 ml of synthesis products were added to 1 ton of washing water before post-harvest storage for tomatoes, apples and citrus fruits. The most successful result was obtained with a 65% increase in fruit shelf life in experiments, in which 750 ml of synthesis product was added to 1 ton of wash water.
 
As a result of this project, after application of liquid synthesis product before storage after
 
fruit and vegetable harvesting, it has been found that the shelf-life of each fruit and vegetable product were increased and losses during storage is reduced or eliminated by 75-80%, There is no problem that could pose a risk for the sale and sustainable management of the product, which is attracted to the product sector and demanded by the market, and which should solve the existing  problem in this context and keep it low compared to its competitors.
 
The success of this study will enable the solution of similar problems in similar sectors that could be solved with similar technology. Reducing stock losses, promoting food safety and industrialization will bring together employment and commercial activities.
 
The aim of this article is to examine the effectiveness of synthetic agricultural products developed using biotechnological methods to prevent the loss of plant-based products to the consumer and to determine the degree of utilization.

Keynote Session:

Meetings International -  Conference Keynote Speaker Pawan Saharan photo

Pawan Saharan

CEO of Biomix Network Ltd, USA

Title: Drug Delivery System and Nano Technology

Biography:

Dr. Saharan has MS in Life Sciences (JNU); Ph.D in medicine (WVU) & Post Doc. (Stanford University).He has several International Publications / Presentations including in Top Scientific Journal Nature from the age of 21.

Nominated for several Global Awards including by New Drug Discovery Programme of Department of Science & Technology (GoI) funded US$ 3 million grant and the best US Scientist Award at age 22 years by AAAS, Washington DC. Dr. Pawan Saharan has been CSO & CEO for large MNC & founder of Biomix Network Inc. USA & India.

Dr. Saharan has global experience in running healthcare industry, including the Pharmaceuticals MNCs and hospitals at the top management level in USA & India

Abstract:

Pawan Saharan has invented Radha108 Nano Peptides that naturally producing new generation antibodies & their  bio similar like Cytokine (Interleukin & Interferon)  by Secretion from Cytotoxic T- cells   of the innate immune system, which was increased by  5 times  due to RADHA 108 Nano Peptide that get absorbed in the blood through buccal mucosa & crosses the Blood Brain Barrier.

Radha108  act on pituitary gland that in turn promotes differentiation of B cells, maturation of macrophages and monocytes & Stimulates production of cytokines IL-1 to IL-11, TNF-α, INF–γ & maturation of immature thymocytes into either helper or suppressor T cells that helps building body's immune system strongly to fight any infection and immune disorders like Asthma, Allergy, URTI, Carcinomas and Type 2 diabetes saving hundreds  of Billions of $ that are spent in treating such ailments with little or no efficacy.

The mode of action of Receptol® is based on API Radha 108 Nano-informational peptides proteins which are active in mitigating cell fusion & docks on Gp120, 180, 160 receptor CD4, CD8 on the cell surface closing entry of virus like foreign antigen and allergens. Radha108 functions as a molecular signaling device which works through receptors on target cell surfaces.

Meetings International -  Conference Keynote Speaker Alexander Zacharia photo

Alexander Zacharia

Odessa National University, Ukraine

Title: Graphite filter-furnace with carbon thread atomizer and chemical modifiers for the electrothermal atomic absorption determination sn, pb, se and as in some of food, biological and environmentals

Biography:

Alexander Zacharia has completed his PhD at the age of 35 years from I.I.Mechnikov Odessa National University. He is the Director of Independent Analytical Chemistry Laboratory “CHEMTEST”, Member of Scientific Council (section on Analytical Chemistry) Ukrainian National Academy of Sciences , Member of Ukrainian Technical Committee TK122 –“ Gases, Liquid and Solids Analysis”. He is the Professor of Odessa National University. He has published more than 52 papers in reputed journals and has been serving as an editorial board member of repute.

Abstract:

According IUPAC recommendation there are several techniques may be used to reduce or eliminate analytical errors resulting from several of interferences in atomic emission (AES) and atomic absorption spectrophotometry (AAS) analysis, including so called the buffer-addition technique. Wherein, in analytical practice of electrothermal (ET) atomic absorption spectrophotometry these spectrochemical buffers, that named as chemical modifiers, are added to both the sample and reference solutions for the purpose of making the measure of the analyte less sensitive to variations in interferent concentration. Additives that may serve in ET AAS analytical practice as spectrochemical buffers are: suppressors, releasers, protective agents, volatilizers. This work are devoted to investigation of analytical effect some of chemical modifiers: Pd(NO3)2, Mg(NO3)2, NH4H2PO4 and their mixtures on the evaporation as well as atomization process for Ge, Sn, Sb and Bi at their direct ET AAS determination in some of materials with atomizer “graphite filter-furnace” (FF) and “carbon thread collector” (CTC)