Cold plasma (CP) technology has found favor in the agricultural industry for changing seed coat structures, increasing the permeability of seed coats and stimulating seed. However, there are still leaking study of mushroom spawns application. The Pleurotus ostreatus (oyster mushrooms) spices spikes in global demand, encouraging Malaysia to take advantage of the lucrative trade. Therefore, the objectives of this study is to evaluate the mushroom spawns wettability for mushrooms germination rate by using CP technology. By using a novel CP pen as shown in Figure, the mushroom spawn will treated by using discharge air. The air generates in a petri dish with a flow rate of 5 L/min by considering three different time exposure (0, 15 and 30 seconds) with 8 kV of supply voltage. Stainless steel rod is a material for electrode pen discharge. The SEM and contact angle analysis were conducting to study the changes in the surface morphology of the plasma treated spawns. The results show an increment of treatment time, increase the mushroom spawn pores and wettability. This creates surface more hydrophilic thus spawn absorbs more water and shorten the germination rate. Based on the results, it can conclude that the CP application can significantly change the spawn wetability and textural