Title: The relationship between MTHFR C677T gene polymorphism and essential hypertension in a sample of an Algerian population of the Oran city

Biography:

Asma Amrani-Midoun is a lecturer. She belongs to Biotechnology Department, University of Oran 1 Ahmed Benbella, Oran, Algeria she is also Faculty of Sciences of Nature and Life, University of Oran 1 Ahmed Benbella, Oran, Algeria. She is the corresponding author of the article “The relationship between MTHFR C677T gene polymorphism and essential hypertension in a sample of an Algerian population of Oran city”.

Abstract

Background:
Many studies have investigated the role of 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism in essential hypertension (EH), but with conflicting results.

Aim:
To determine the eventual association between 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism and hypertension in a sample of the Algerian population from the Oran city.

Methods:
A case-control study has been performed in 154 subjects including 82 hypertensives defined as subjects with elevated systolic blood pressure SBD140mmHg and or sustained diastolic blood pressure DBP≥90mmHg, and 72 normotensive subjects. Polymerase chain reaction (PCR) combined with restrictive fragment length polymorphism (RFLP) was used to detect the MTHFR C677T variant.

Results:
We observe no significant differences between allelic and genotypic frequencies between cases and controls for C677T polymorphism (OR=1.51, 95% CI= 0.89-2.56, P=0.13). Analyses adjusted for age, sex and body mass index improved the association level, though the association was still not significant (30% vs. 22%, OR=1.75, 95% CI= 0.95-3.24, P=0.07).

Conclusion:
This work showed that genetic polymorphism related to the MTHFR gene (C677T) is not associated with the risk of hypertension in this sample of the Algerian population. Larger case-control samples are required to clearly assess the role of this genetic variant in EH.