Title: Discrimination of red blood cells of different pathological states by statistical analysis of physical parameters, obtained with biosensing technology during microcirculatory

Biography:

Bruno Le Pioufle, PhD from University Paris XI, is Professor at ENS Paris-Saclay, within the University Paris-Saclay. He is the Director of Institut d’Alembert. He is author of more than 70 papers in scientific journals

Abstract

Red blood cell (RBC) genetic disorders affect millions of peoples in the world. Abnormal hemoglobin (HbS) is produced and fills the denuclearized RBC. Under hypoxic condition, HbS polymerizes and RBC with a diameter of 8µm loses its elasticity and deformability to pass through the blood microcapillaries network. Such disorder can be found for example in Sickle Cell Disease (SCD), and might induce vaso-occlusive and organs dysfunction. For the abnormal RBCs detection, bioimpedance measurement of cells injected into a microfluidic device is proposed. Electrically monitoring the transit of RBC in the microfluidic network, comprising fluidic restrictions, the RBCs of different pathological states can be discriminated. The device was made in Polydimethylsiloxane (PDMS) casted thanks to soft lithography technology.