Title: Exploring therapeutic potential of Lecith potentiates the protective effect of rofecoxib and berberine against ICV-STZ induced memory impairment in a rat model of sporadic dementia of Alzheimers type

Biography:

Arti Singh has completed her PhD at the age of 30 years from Panjab University and Postdoctoral Studies from Department of Physiology, School National University of Singapore, Singapore. Currently She is working as Associate Professor in the Department of Pharmacology, ISF College of Pharmacy Moga-142001, Punjab INDIA. She has published more than 15 papers in reputed journals.

Abstract

Lecithin (phosphatidylcholine analogue) is a good source of choline in the treatment of cognitive dysfunction. Rofecoxib, a selective cox-2 inhibitor belongs to non-steroidal anti-inflammatory class of drugs. Berberine, an isoquinoline alkaloid is reported to exhibit antioxidant effect and cholinesterase (ChE) inhibitor activity. Aim is to investigate the neuroprotective potential of choline enrichment carrier lecithin along with rofecoxib and berberine in ICV-STZ induced cognitive dysfunction in rats. Animals received single bilateral ICV injections of STZ (3 mg/kg). Drug galantamine (2 mg/kg) used as, standard care and lecithin (100, 200 & 400 mg/kg), berberine (50 & 100 mg/kg) and rofecoxib (10 & 20 mg/kg) was administered for a period of 21 days. Various neurobehavioral parameters bodyweight, locomotor activity, Morris water maze, Elevated plus maze was evaluated, followed by biochemical (oxidative stress parameters), AChEs level, molecular (TNF-α level), mitochondrial respiratory enzyme complexes (I-IV) and histopathological (H&E staining) evaluations. In this study, we found that single bilateral ICV-STZ administration significantly impaired cognitive performance on MWM test, increased oxidative stress markers (raised lipid peroxidation, nitrite concentration, reduced glutathione, catalase activity), increased AChE level and neuroinflammation (increased TNF-α levels) and causes mitochondrial dysfunction and histopathological alterations as compared to sham. Chronic combination treatment of lecithin (200 & 400 mg/kg) with berberine (50 & 100 mg/kg) and rofecoxib (10 & 20 mg/kg) for 21days attenuated spatial learning and memory (MWM performance) with reduced transfer latency and increased TSTQ, reduced AChEs activity, oxidative damage, TNF-α level, restored mitochondrial respiratory enzyme complex (I, II, III, IV) activities and reduced histopathological alterations in ICV-STZ treated rats as compared to their effect alone. The present study suggests that Lecithin potentiates the protective potential of berberine and rofecoxib against single bilateral ICV-STZ induced cognitive dysfunction in rats